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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2539-2550
DOI: https://doi.org/10.20546/ijcmas.2019.802.296


Molecular Cloning and Characterization of Coat Protein Gene of Banana bract mosaic virus Affecting Banana cv. Mysore Poovan (Aab)
G. Darshan1, C.K. Anitha2*, S. Manjesh1 and P.S. Abida3
1Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, Thrissur, India
2Department of Plant Pathology, College of Horticulture, Kerala Agricultural University, Thrissur, India
3Department of Plant Breeding and Genetics, Regional Agricultural Research Station, Pattambi, Kerala, India
*Corresponding author
Abstract:

Banana bract mosaic disease (BBrMD) is one of the most important viral diseases of banana which leads to a yield reduction. We have identified banana bract mosaic virus (BBrMV) in banana plants growing in the regions of southern India based on symptomatology, sequence homology, and Serodiagnostic assays. The viral sequence encoding the coat protein was specifically amplified by RT-PCR (Reverse Transcriptase – Polymerase chain reaction) using specific primers bordering the Coat Protein (CP) gene. The unique amplified product thus obtained was cloned into the pGEM-T vector and the authenticity of the cloned gene verified by colony PCR. The nucleotide sequences and the deduced amino acid sequences were compared with the other BBrMV isolates and found to be identical at both the nucleotide and amino acid sequence level of other isolates with 99 to 96 per cent and 95 to 83 per cent respectively. The phylogenetic analysis by the alignment of CP gene sequences of selected 22 isolates also revealed that the present isolate was more similar to KER2 (Kasaragod) isolate. The recombinant clones developed in the present study could be applied in serodiagnosis and genetic engineering. This could be also used as disease diagnostic probes for more sensitive molecular diagnostic techniques like Nucleic acid spot hybridization.


Keywords: Banana bract mosaic virus, Coat Protein gene, Mysore Poovan, Cloning, ELI
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How to cite this article:

Darshan, G., C.K. Anitha, S. Manjesh and Abida, P.S. 2019. Molecular Cloning and Characterization of Coat Protein Gene of Banana bract mosaic virus Affecting Banana cv. Mysore Poovan (Aab).Int.J.Curr.Microbiol.App.Sci. 8(2): 2539-2550. doi: https://doi.org/10.20546/ijcmas.2019.802.296