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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2508-2517
DOI: https://doi.org/10.20546/ijcmas.2019.802.292


Mitochondria COI-Based Molecular Characterization and Genetic Analysis of the Fenazaquin Selected Resistant Strain of Two-Spotted Spider Mite, Tetranychus urticae Koch
Rakesh Kumar Sharma1*, Manmeet Brar Bhullar2 and Satnam Singh3
1Dr D R Bhumbla RRS, Ballowal Saunkhri, Punjab, India
2Department of Entomology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
3RRS PAU, Faridkot, India
*Corresponding author
Abstract:

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) has emerged as an important agricultural pest in a wide range of outdoor and protected crops worldwide. Fenazaquin is METI-acaricide which is used extensively and frequently for the management of this mite has resulted in the development of resistance. So, present studies were conducted to investigate molecular characterization and genetic analysis based on mtCOI sequence between fenazaquin resistant and susceptible population of T. urticae as very limited information is available regarding mutation/variability in genes involved in imparting resistance. Fenzaquin resistance population was developed in the laboratory by giving selection pressure with fenazaquin for 15 generations leading to 166.49 fold resistance when compared with susceptible population. Molecular characterization of resistant and susceptible population revealed no changes in genes structure of mtCOI in the resistant compared to the susceptible population. In our studies high level of resistance to fenazaquin didn’t show any change in the amino acid sequence of COI region of resistant and susceptible populations Thus results revealed mtCOI as a stable gene which is least influenced by acaricide resistance.


Keywords: Tetranychus urticae, METI acaricides, Resistant, mt COI
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How to cite this article:

Rakesh Kumar Sharma, Manmeet Brar Bhullar and Satnam Singh. 2019. Mitochondria COI-Based Molecular Characterization and Genetic Analysis of the Fenazaquin Selected Resistant Strain of Two-Spotted Spider Mite, Tetranychus urticae Koch.Int.J.Curr.Microbiol.App.Sci. 8(2): 2508-2517. doi: https://doi.org/10.20546/ijcmas.2019.802.292