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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:2, February, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2404-2409
DOI: https://doi.org/10.20546/ijcmas.2019.802.280


Virulence of Steinernima and Heterorhabditis against black Cutworm, Agrotis segetum (Lepidoptera: Noctuidae) in Potato Crop
Neelam Kumari1, Deepak C. Kalia1 and V.K. Chandla2
1Department of Bio-sciences, Himachal Pradesh University Shimla, India
2Department of Plant Protection, ICAR- CRRI Shimla, India
*Corresponding author
Abstract:

The common cutworm (turnip moth) Agrotis segetum (Denis & Schiffermuller) is nearly cosmopolitan insect pest of potato. The efficacy of the entomopathogenic nematodes (EPN) Steinernema carpocapsae and Heterorhabditis indica was evaluated against different developmental stages of A. segetum under laboratory conditions. In laboratory studies, significant mortality of second instar larvae was recorded by H. indica (73.3-100 per cent) and 63.3-100 per cent by S. carpocapsae after 48 hrs of treatment at EPNs suspension of 50-300 IJs/ml (1 ml per larva). In the preliminary bioassay study, all the tested EPNs were capable to cause maximum mortality within three to five days of treatment. The highest mortality (100 per cent) of fourth instar larvae and pupae was observed at more than 200 IJs/ml nematodes suspension of S. carpocapsae and H. indica after five days of treatment. At nematodes suspension 50 IJs/ml, 56.7 per cent mortality of fourth instar was recorded by S. carpocapsae and 76.7 per cent by H. indica after five days of treatment. The pupae of A. segetum were less susceptible to EPNs infections as compared to larvae due to reduced number of portal entries.


Keywords: Steinernima and Heterorhabditis black Cutworm, Agrotis segetum

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How to cite this article:

Neelam Kumari, Deepak C. Kalia and Chandla, V.K. 2019. Virulence of Steinernima and Heterorhabditis against black Cutworm, Agrotis segetum (Lepidoptera: Noctuidae) in Potato Crop.Int.J.Curr.Microbiol.App.Sci. 8(2): 2404-2409. doi: https://doi.org/10.20546/ijcmas.2019.802.280
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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