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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
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Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2314-2325
DOI: https://doi.org/10.20546/ijcmas.2019.802.270


EST-SSR based Genetic Divergence and Prediction of Heterotic Combinations of Hybrid Parents in Pigeonpea
B.R. Raghu1*, R.S. Raje2, N.K. Singh3, K.B. Saxena4, D.A. Chauhan5, S.A. Acharya6, A.N. Patil7, J.B. Patil6, K.B. Wanzari7 and D. Khare8
1ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand, 263 601, India
2Division of Genetics, ICAR-Indian Agricultural Research Institute, N. Delhi, India
3ICAR-National Research Center on Plant Biotechnology, N. Delhi, India
4Principal scientist (ICRISAT, Retd.), NMC housing complex, Al Ain, Abu Dhabi, UAE
5Navsari Agricultural University, Navsari (Gujarat), India
6SDAU, Sardakrishinagar, Dantiwada, Palanpur, Banaskantha (Gujarat), India
7PDKVV, Akola, Maharastra, India
8JNKVV, Jabalpur, India
*Corresponding author
Abstract:

Cytoplasmic-genic male sterile based F1 hybrid is a revolutionary approach to break yield stagnation in pigeonpea. Hybrid vigor is positively correlated to genetic distance and allelic divergence of the parents. This study estimated genetic divergence of 8 ‘A’ lines, 2 ‘B’ lines and 19 ‘R’ lines of A2 and A4 cytoplasmic sources, and predicted best parental combinations using molecular markers. Among 390 EST-SSR markers screened, only 60 markers found polymorphic indicating very low degree of polymorphism (15.4%). Average allelic count was 2.26 with a range of 2-4 per loci. Mean PIC was 0.358 with a range of 0.5885 (ASSR236) and 0.1780 (ASSR9). Cluster and factorial analyses were performed based on marker dissimilarity matrix. The best parental combinations identified from the present study were, ICPA2047 X ICPR4013; ICPA2043 X ICPR4013 for A4 cytoplasm, and GT290A X GTR9, GT33A X GTR9, MS01A X GTR 9, MS10A X GTR9 for A2 cytoplasm in pigeonpea.


Keywords: Cytoplasmic-genetic-male-sterility, Genetic-diversity, Hybrid-breeding, Microsatellite- markers, Pigeonpea
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How to cite this article:

Raghu, B.R., R.S. Raje, N.K. Singh, K.B. Saxena, D.A. Chauhan, S.A. Acharya, A.N. Patil, J.B. Patil, K.B. Wanzari and Khare, D. 2019. EST-SSR based Genetic Divergence and Prediction of Heterotic Combinations of Hybrid Parents in Pigeonpea.Int.J.Curr.Microbiol.App.Sci. 8(2): 2314-2325. doi: https://doi.org/10.20546/ijcmas.2019.802.270