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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2124-2134
DOI: https://doi.org/10.20546/ijcmas.2019.802.246


Detection of Brucellosis by Serological Techniques in Bovines
Tejal Walunj1*, Prashant Mhase1, Sujata Bhave2, Dushyant Muglikar1 and Mrunalini Pawde1
1Department of Veterinary Microbiology
2Department of Veterinary Public Health, KNP College of Veterinary Science, Shirwal, Satara, India
*Corresponding author
Abstract:

Brucellosis is an important infectious disease of livestock affecting a wide range of animal species including human and is characterized by abortions, fetal death, reproductive tract complications and arthritis in animals with great losses. Early precise diagnosis with more sensitive and specific test is a very important for its control and eradication. For the diagnosis of brucellosis different serological tests are used widely, but often they come with certain disadvantages like longevity and lack specificity and gold standard isolation cultural test requires special biosecurity facilities and poses a danger of infection. Hence, highly sensitive genus specific molecular techniques are preferred. Hence present study deals with the screening of farms having clinical brucellosis with serological tests; RBPT and iELISA. It was concluded that brucellosis was more in animals belonging to Ahmednagar than Pune, was higher in buffaloes than in cattle, and was more in the aborted than In-contact animals and higher in animals of organized farms than unorganized farms. The seropositivity was marginally higher in ELISA-2 kit than Indigenous ELISA-1 followed by RBPT.


Keywords: Brucellosis, Rose bengal plate test, ELISA, Cattle, buffalo
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How to cite this article:

Tejal Walunj, Prashant Mhase, Sujata Bhave, Dushyant Muglikar and Mrunalini Pawde. 2019. Detection of Brucellosis by Serological Techniques in Bovines.Int.J.Curr.Microbiol.App.Sci. 8(2): 2124-2134. doi: https://doi.org/10.20546/ijcmas.2019.802.246