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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 1625-1631
DOI: https://doi.org/10.20546/ijcmas.2019.802.191


Response of Pollen Viability in Gladiolus (Gladiolus grandiflorusL.) to Storage Method and Period
Simarjit Kaur* and K.K. Dhatt
Departmetnt of Floriculture, Punjab Agricultural University, Ludhiana (141004), Punjab, India
*Corresponding author
Abstract:

The experiment was conducted in Punjab Agricultural University, Ludhiana, Punjab during the season of 2016 and 2017, in order to determine the pollen viability response to storage method and period in Gladiolus (Gladiolus grandiflorus L.). Gladiolus var. Yellow stone used as male parent for pollen collection. The corms of gladiolus were planted during October–December to obtained fresh pollen for research. Pollen grains of variety Yellow stone packed in plastic vials were stored in desiccator at ambient temperature, desiccator with CaCl¬2 at ambient temperature, desiccator under refrigerator, desiccator with CaCl2 under refrigerator (5˚C), lower cabin under refrigerator (5˚C), freezer(-4°C) for 16 weeks from January to April. Pollen stored under different storage methods was used for determination of pollen viability. Maximum pollen viability was recorded under freezer i.e. 82.87%. Pollen stored under freezer retained pollen viability for longer duration and it was recorded 85.09% after 11weeks and pollen viability decreases to 49.58% after 16 weeks of storage. Pollen of the gladiolus can be stored for longer duration under freezer condition up to 11 weeks of storage without any serious loss of viability. The pollen of gladiolus can be stored in freezer (-4˚C) up to 2½ month for obtaining good pollen viability.


Keywords: Gladiolus grandiflorus, Pollen storage, Viability, Desiccator, Freezer
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How to cite this article:

Simarjit Kaur and Dhatt, K.K. 2019. Response of Pollen Viability in Gladiolus (Gladiolus grandiflorusL.) to Storage Method and Period.Int.J.Curr.Microbiol.App.Sci. 8(2): 1625-1631. doi: https://doi.org/10.20546/ijcmas.2019.802.191