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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 1572-1579
DOI: https://doi.org/10.20546/ijcmas.2019.802.184


Molecular Detection and Characterization of Niger [Guizotia abyssinica (L.f.) Cass] Phyllody Phytoplasma
Mahalingappa Bandakkanavara1*, H. A. Prameela1, Santosh Mali2, S. Basavaraj1, Manjunath, S. Hurakadli1, Kedarnath1 and K.T. Rangaswamy1
1Department of Plant Pathology, College of Agriculture, UAS, GKVK, Bengaluru-560065
2Department of Agricultural Entomology, College of Agriculture, UAS, GKVK, Bengaluru-560065, India
*Corresponding author
Abstract:

Niger [Guizotia abyssinica (L.f.) Cass.] is one of the important minor oilseed crops in India. Phyllody disease on niger noticed for the first time at Main Research Station, Hebbal, Bengaluru, Karnataka. The molecular detection and characterization of the phytoplasma causing niger phyllody was investigated during Kharif 2016. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by polymerase chain reaction (PCR) from infected samples, as well as by sequencing and phylogenetic analysis. The molecular detection by using nested PCR phytoplasma specific universal primers R16F2n/R16FR2 revealed the amplification of phytoplasmal specific PCR product of 1.2 kb fragment corresponding to the 16S rDNA. The16S rDNA sequence of niger phyllody phytoplasma had maximum nucleotide identity of 90 per cent with the 16S rDNA sequence of Cymbopogon citratus white leaf phytoplasma (KF773150.1), Alfalfa witches'-broom Phytoplasma Mes 38(KT943964.1), Sesamum indicum phyllody Phytoplasma (KY547787.1) and Vigna radiata phyllody NDL (KY439871.1).


Keywords: Molecular detection, Niger, Phyllody, Phytoplasma
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How to cite this article:

Mahalingappa Bandakkanavara, H. A. Prameela, Santosh Mali, S. Basavaraj, Manjunath, S. Hurakadli, Kedarnath and Rangaswamy, K.T. 2019. Molecular Detection and Characterization of Niger [Guizotia abyssinica (L.f.) Cass] Phyllody Phytoplasma.Int.J.Curr.Microbiol.App.Sci. 8(2): 1572-1579. doi: https://doi.org/10.20546/ijcmas.2019.802.184