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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 1564-1571
DOI: https://doi.org/10.20546/ijcmas.2019.802.183


Molecular Detection and Characterization of Papaya Ring Spot Virus (PRSV) Disease in Jorhat District of Assam, India
Shankar Hemanta Gogoi*1, P.D. Nath1, N. Thakuria2, S. Gogoi2, B. Das2, N. Deka3 and K. Raj3
1Department of Plant Pathology, College of Agriculture, Assam Agricultural University, Jorhat-13, Assam, India
2Institute of Science and Technology, Guwahati University, Guwahati-781014, Assam, India
3Sikkim Manipal Institute of Medical Sciences, Sikkim-737102, India
*Corresponding author
Abstract:

Papaya ring spot virus (PRSV) is the most destructive disease of Papaya, responsible for yield reduction of Papaya all over the world. PRSV exhibited different types of symptoms on Papaya plant in the field like ringspot , vein clearing, vein banding, chlorosis of younger leaves, filiformy, shoestring, mosaic, mottling, stunted growth etc. Molecular diagnosis of PRSV was done with the help of gene specific primers through PCR. Gel electrophoresis results show a 300bp clear band which confirms the presence of PRSV in the plants. Sequencing was done and phylogenetic tree was made. Sequencing results showed 89-97 percent identities with other PRSV isolates present in NCBI Genebank. Phylogenetic analysis reveals that the PRSV Jorhat isolate is closely similar to that Assam isolate Accession no. KC149500.


Keywords: Papaya ring spot virus (PRSV), Symptoms, PCR, NCBI, Phylogeny
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How to cite this article:

Shankar Hemanta Gogoi, P.D. Nath, N. Thakuria, S. Gogoi, B. Das, N. Deka and Raj, K. 2019. Molecular Detection and Characterization of Papaya Ring Spot Virus (PRSV) Disease in Jorhat District of Assam, India.Int.J.Curr.Microbiol.App.Sci. 8(2): 1564-1571. doi: https://doi.org/10.20546/ijcmas.2019.802.183