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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Int.J.Curr.Microbiol.App.Sci.2019.8(2): 1080-1088
DOI: https://doi.org/10.20546/ijcmas.2019.802.127


Genetic Response of Sugarcane (Saccharum officinarum L.) Genotypes to Varying Concentrations of Cytokinins for in vitro Shoot Multiplication
Suresh Yadav1*, T.E. Nagaraja2, H.C. Lohithaswa2, K.V. Shivakumar2, Poonam Kumari Yadav3, Poonam Yadav4, Ganpat Louhar5 and Jagdish Yadav6
1Division of Genetics, IARI, New Delhi, India
2Department of Genetics and Plant Breeding, V.C. Farm, Mandya, University of Agricultural Sciences, Bangalore, India
3Department of soil science and agricultural chemistry, SKN College of Agriculture (SKNAU), Jobner, 303329 Jaipur, India
4Department of Livestock Production and Management, SKN College of Agriculture (SKNAU), Jobner, 303329 Jaipur, India
5Division of soil science and agricultural chemistry, IARI, New Delhi, India
6Division of Plant Pathology, Indian Agricultural Research Institue, New Delhi, 110012, India
*Corresponding author
Abstract:

Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing 1.0 mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, 0.5, 1, 1.5 and 2 mg/l) and five levels of kinetin (0, 0.25, 0.5, 1.0 and 1.5 mg/l) in completely randomized design with 5x5x2 factorial treatment combinations. Analysis of variance (ANOVA) showed that the interaction effects of 6-benzlyaminopurine (6-BAP), kinetin and the sugarcane genotypes on number of shoots per explant, shoot length, and chlorophyll content was highly significant (p< 0.001), except for number of leaves. Multiplication of the cultures was obtained by using various combinations of 6-BAP and kinetin in MS medium. The optimum multiplication for genotype Co 86032 was obtained when MS media supplemented with 1.0 mg/l 6-BAP and 0.5 mg/l kinetin as this genotype produced 32.5 shoots per explant with 6.32 cm shoot length, 2.83 leaves and chlorophyll content of 20.78 mg/g. best performance of CoVC-18061 with respect to number of shoot per explant (27.75), shoot length (7.03) with number of leaves (2.81) and chlorophyll content (22.83 mg/g) was obtained on MS medium fortified with combination of 1.5 mg/l BAP and 0.5 mg/l kinetin after 30 days of culture transferred to multiplication media. The performance of genotype for all characters was very poor in MS medium amended with other combinations. Thus, the optimized protocol is useful for rejuvenation, rapid in vitro propagation and production of large quantity of quality plants.


Keywords: In vitro Shoot multiplication, Sugarcane, 6- Benzylamminopurine (6-BAP), Kinetin
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How to cite this article:

Suresh Yadav, T.E. Nagaraja, H.C. Lohithaswa, K.V. Shivakumar, Poonam Kumari Yadav, Poonam Yadav, Ganpat Louhar and Jagdish Yadav. 2019. Genetic Response of Sugarcane (Saccharum officinarum L.) Genotypes to Varying Concentrations of Cytokinins for in vitro Shoot Multiplication.Int.J.Curr.Microbiol.App.Sci. 8(2): 1080-1088. doi: https://doi.org/10.20546/ijcmas.2019.802.127