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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 8, Issue:2, February, 2019
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2019.8(2): 1062-1067
DOI: https://doi.org/10.20546/ijcmas.2019.802.125


Development of Next Generation Sequencing Protocols for dsRNA Viruses
Sushila Maan1*, Deepika Chaudhary2, Nitish Bansal2, Bhanita Devi2 and Narender S. Maan2
1Department of Animal Biotechnology
2College of Veterinary Sciences, LalaLajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
*Corresponding author
Abstract:

Next generations sequencing (NGS) is a very important tool to identify pathogens in different forms i.e. viruses, bacteria etc. This helpful in decode life mysteries, detect pathogens and improve life qualities. Next generation sequencing/Massively parallel sequencing it allows millions of sequencing reactions to be carried out in parallel due to this accuracy and sequencing rate is high as compare to other sequencing methods. In this communication we tried to develop a protocol for dsRNA viruses for MIseq platform.


Keywords: dsRNA, cDNA synthesis, Tagmented DNA, Denaturation

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How to cite this article:

Sushila Maan, Deepika Chaudhary, Nitish Bansal, Bhanita Devi and Narender S. Maan. 2019. Development of Next Generation Sequencing Protocols for dsRNA Viruses.Int.J.Curr.Microbiol.App.Sci. 8(2): 1062-1067. doi: https://doi.org/10.20546/ijcmas.2019.802.125
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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