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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 1062-1067
DOI: https://doi.org/10.20546/ijcmas.2019.802.125


Development of Next Generation Sequencing Protocols for dsRNA Viruses
Sushila Maan1*, Deepika Chaudhary2, Nitish Bansal2, Bhanita Devi2 and Narender S. Maan2
1Department of Animal Biotechnology
2College of Veterinary Sciences, LalaLajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
*Corresponding author
Abstract:

Next generations sequencing (NGS) is a very important tool to identify pathogens in different forms i.e. viruses, bacteria etc. This helpful in decode life mysteries, detect pathogens and improve life qualities. Next generation sequencing/Massively parallel sequencing it allows millions of sequencing reactions to be carried out in parallel due to this accuracy and sequencing rate is high as compare to other sequencing methods. In this communication we tried to develop a protocol for dsRNA viruses for MIseq platform.


Keywords: dsRNA, cDNA synthesis, Tagmented DNA, Denaturation
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How to cite this article:

Sushila Maan, Deepika Chaudhary, Nitish Bansal, Bhanita Devi and Narender S. Maan. 2019. Development of Next Generation Sequencing Protocols for dsRNA Viruses.Int.J.Curr.Microbiol.App.Sci. 8(2): 1062-1067. doi: https://doi.org/10.20546/ijcmas.2019.802.125