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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 855-868
DOI: https://doi.org/10.20546/ijcmas.2019.802.097


Phytochemical and Thin Layer Chromatographic Evaluation of Swertia chirayita Buch.-Hams. Ex Wall at Different Developmental Stages
Garima Kumari1*, Ashish Guleria2 and Jasmeen Kaur3
1Department of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, India
2Department of Applied Sciences, Women Institute of Technology, Dehradun, India
3Department of vegetable sciences, PAU, Ludhiana, India
*Corresponding author
Abstract:

Swertia chirayita (family Gentianaceae), imperative medicinal plant in Indian system of medicine known for its bitter principles. However, due to its high demand and scarcity due to its extinction, it is being frequently adulterated with other species of Swertia which are more readily available. At present the similar species of Swertia are marketed as ‘chirayita’ which is affecting the potency of the drug. Our studies focused on evaluating Swertia chirayita by comparison of its physicochemical characteristics and TLC fingerprinting profile in Chloroform:Methanol:Water as solvent employing amarogentin as a reference marker to distinguish the crude herb chirayita from its adulterants. The study revealed methanol soluble fraction of Swertia species showed presence of amarogentin (major chemical constituent) in the raw material taken and thus can be further micropropagated on large scale to meet industrial demand.


Keywords: Swertia, Thin layer chromatography, Pluriannual, Medicinal, Micropropagated, Phytochemical
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How to cite this article:

Garima Kumari, Ashish Guleria and Jasmeen Kaur. 2019. Phytochemical and Thin Layer Chromatographic Evaluation of Swertia chirayita Buch.-Hams. Ex Wall at Different Developmental Stages.Int.J.Curr.Microbiol.App.Sci. 8(2): 855-868. doi: https://doi.org/10.20546/ijcmas.2019.802.097