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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 195-202
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.021


Role of Serology in Diagnosis of Human Hydatidosis: Experience from a Tertiary Care Hospital
Mridula Madiyal1*, Barnini Banerjee2, Anson Kunjumon George2, Vandana Kalwaje Eshwara2, S.S. Prasad2 and Kiran Chawla2
1Department of Microbiology, Kasturba Medical College, Manipal, Manipal University, India
2Department of Surgery, Kasturba Medical College, Manipal, Manipal, India
*Corresponding author
Abstract:

Hydatid disease is an important emerging neglected disease worldwide, with significant geographic variation in seroprevalance. The disease is commonly diagnosed on basis of clinical suspicion, imaging and serology. This study was conducted to evaluate our experience with serology in diagnosing hydatid disease in terms of identifying the numbers of patients testing positive for antibodies against Echinococcus, and to study their clinical profile. This retrospective study included all patients who tested for anti-Echinococcus IgG antibodies by ELISA in the years 2014-2015. The clinical characteristics of seropositive patients were contrasted with seronegative patients, and the test results were correlated with radiological findings and, where performed, with histopathological studies. Analysis was performed on SPSS (IBM, Inc). Chi-square test was used to statistically compare between patient groups. Out of 54 patients evaluated by serology for Echinococcosis during the study period, Fourteen (26%) were seropositive. The two groups were comparable with respect to age, gender and most hematological parameters, excepting eosinophilia, which was more frequent among seropositive patients (28.6% vs. 2.5%; p=0.01). Alkaline phosphatase also tended to be higher among seropositives (42.9% vs 20%; p=0.09). Liver was the most frequent site involved (12 patients; 86%). Of the 29 patients who also underwent biopsy, all the seven patients with positive biopsy were seropositive, suggesting 100% sensitivity. The specificity was 81.8%, but this might be a lower estimate due to potentially high rate of false-negative biopsies, as all the seropositive patients also had imaging features suggestive of disease. The positive and negative predictive values were 63.6% and 100%, respectively. ELISA for detection of anti-Echinococcus antibodies is a simple serological test that helps in correlation with imaging finding in the diagnosis and subsequent management of hydatid disease.


Keywords: Cystic Echinococcosis, Echinococcus granulosus,Hydatid disease, Seropositivity, Parasitic zoonoses.
 
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How to cite this article:

Mridula Madiyal, Barnini Banerjee, Anson Kunjumon George, Vandana Kalwaje Eshwara, S.S. Prasad and Kiran Chawla. 2016. Role of Serology in Diagnosis of Human Hydatidosis: Experience from a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 5(12): 195-202. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.021