National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present study was conducted to evaluate the anti-apoptotic effect of caspase inhibitor (Z-IETD-FMK) supplementation in buffalo bull semen. The Z-IETD-FMK was supplemented with Tris egg yolk extender @ 2, 4, 6, 10 and 20 µM. The pre-freeze and post thaw samples were evaluated in terms of % individual motility, % viability, % HOS reactive sperms, status of mitochondrial membrane potential and status of sperm membrane phosphatidylserine. There was no significant effect (P>0.05) of Z-IETD-FMK treatment on sperm motility (%), sperm viability (%) and percent sperm with active mitochondria at pre-freeze and post-thaw stages. However, there was improvement in terms of % HOS reactive sperms and % sperms with low PLA activity in higher supplementation doses (20 µM) of Z-IETD-FMK in post freeze semen samples as compared to control. Thus Z-IETD-FMK shows anti-apoptotic effect in higher doses.
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