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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 516-524
DOI: https://doi.org/10.20546/ijcmas.2019.802.059


Effect of Z-IETD-Fmk (Caspase Inhibitor) Supplementation on Apoptosis Like Changes Developed in Buffalo Bull Sperm during Cryopreservation
Jasmer Dalal1*, AjeetKumar2, M. Honparkhe1 and P.S. Brar1
1Department of Veterinary Gynaecology and Obstetrics, Lala Lajpat University of Veterinary and Animal University, Hisar, 125004, India
2Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, Punjab, India
*Corresponding author
Abstract:

Present study was conducted to evaluate the anti-apoptotic effect of caspase inhibitor (Z-IETD-FMK) supplementation in buffalo bull semen. The Z-IETD-FMK was supplemented with Tris egg yolk extender @ 2, 4, 6, 10 and 20 µM. The pre-freeze and post thaw samples were evaluated in terms of % individual motility, % viability, % HOS reactive sperms, status of mitochondrial membrane potential and status of sperm membrane phosphatidylserine. There was no significant effect (P>0.05) of Z-IETD-FMK treatment on sperm motility (%), sperm viability (%) and percent sperm with active mitochondria at pre-freeze and post-thaw stages. However, there was improvement in terms of % HOS reactive sperms and % sperms with low PLA activity in higher supplementation doses (20 µM) of Z-IETD-FMK in post freeze semen samples as compared to control. Thus Z-IETD-FMK shows anti-apoptotic effect in higher doses.


Keywords: Apoptosis, Buffalo bull, Caspase inhibitor, Cryopreservation, Z-IETD-FMK
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How to cite this article:

Jasmer Dalal, AjeetKumar, M. Honparkhe and Brar, P.S. 2019. Effect of Z-IETD-Fmk (Caspase Inhibitor) Supplementation on Apoptosis Like Changes Developed in Buffalo Bull Sperm during Cryopreservation.Int.J.Curr.Microbiol.App.Sci. 8(2): 516-524. doi: https://doi.org/10.20546/ijcmas.2019.802.059