Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 500-508
DOI: https://doi.org/10.20546/ijcmas.2019.802.057


A Comparative Study of Blood Culture, Widal Test and Immunochromatographic Assay for Rapid Diagnosis of Typhoid Fever in a Tertiary Care Centre
Maya Kumar1*, G. Velvizhi1, G. Sucilathangam2 and C. Revathy1
1Department of Microbiology, Tirunelveli Medical College, Tirunelveli - 627011, Tamil Nadu, India
2Department of Microbiology, Government Theni Medical College, Theni - 625512, Tamil Nadu, India
*Corresponding author
Abstract:

Typhoid fever continues to be a major public health problem and the emergence of antimicrobial resistance by Salmonellae typhi adds to the complexity in treating the patients. Salmonella enterica subspecies enterica serovar typhi, the human specific, causative agent of typhoid fever, is one of the most common infectious diseases in developing countries like India 1. Widal test is associated with numerous limitations, but is still considered and extensively used as the diagnostic tool in our area. The bacteriological identification by blood culture is the best confirmative test of Typhoid fever. The aim of the study was to determine the reliability of Immunochromatographic test for the early diagnosis of typhoid fever when compared to the Widal test. The present study was carried out in Tirunelveli Medical College and Hospital, Tirunelveli for a period of one year from June 2017 to July 2018. A total number of 100 clinically suspected Typhoid fever patient's blood samples were taken and blood culture, widal test and Immunochromatographic tests were done. A total of 14 Salmonella typhi were isolated from 100 clinically suspected typhoid cases. A total of 47 samples were tested positive in Widal test. Out of this only one sample was positive for blood culture (True positivity rate – 2.1% and True negativity rate – 75.4%). Out of 26 samples positive for IgM in ICT, 10 samples were positive for blood culture (True positivity rate – 38.5%). Out of 74 samples that were negative for IgM, only 4 samples were found to be positive for blood culture (True negativity rate – 94.6%). In conclusion, the study implies that rapid ICT tests offers increased sensitivity, rapidity and simplicity over blood culture and Widal test, and can be used as a reliable, alternate early diagnostic tool to the most commonly used serological tests.


Keywords: Typhoid fever, Widal test, Immunochromatographic Test, Blood culture, Salmonella typhi
Download this article as Download

How to cite this article:

Maya Kumar, G. Velvizhi, G. Sucilathangam and Revathy, C. 2019. A Comparative Study of Blood Culture, Widal Test and Immunochromatographic Assay for Rapid Diagnosis of Typhoid Fever in a Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 8(2): 500-508. doi: https://doi.org/10.20546/ijcmas.2019.802.057