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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 481-485
DOI: https://doi.org/10.20546/ijcmas.2019.802.054


Studies on the Effect of Various Sterilization Procedures for in vitro Propagation of Carnation (Dianthus caryophyllus L.)
Bharti Gautam*, Puja Sharma, Y.C. Gupta, Anil Handa, Manisha Thakur and Priyanka Sharma
Department of Floriculture and Landscape Architecture, Dr Y.S.Parmar University of Horticulture and Forestry, Nauni, Solan (HP)-173230, India
*Corresponding author
Abstract:

The present investigation entitled, “Studies on the effect of various sterilization procedures for in vitro propagation Of Carnation (Dianthus caryophyllus L.) was carried out at the Plant Tissue Culture Laboratory of Department of Floriculture and Landscape Architecture, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan (HP) as a refinement in already existing protocol to find suitable and less hazardous surface sterilization chemicals than Mercuric Chloride which is one of the most widely used surface sterilant in micropropagation of carnation. The use of this chemical is being prohibited because of the presence of heavy metal ions in it, causing environment hazards. The necessity to consider an alternative surface sterilization agent is therefore obvious. The experiments were laid out in a Completely Randomized Design (factorial) consisting of two cultivars i.e. ‘Parendillo’ and ‘Yellow Star’. Treatment of explants with 5 % Calcium Hypochlorite is suggested as a potential substitute for Mercuric Chloride. 100 % uncontaminated growing cultures in cv. ‘Parendillo’ and ‘Yellow Star’ were obtained with 5 % Calcium Hypochlorite treatment of explants for 10 and 15 minutes, respectively.


Keywords: In vitro, Surface sterilization, Heavy metals, Hazardous, Uncontaminated growing cultures
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How to cite this article:

Bharti Gautam, Puja Sharma, Y.C. Gupta, Anil Handa, Manisha Thakur and Priyanka Sharma. 2019. Studies on the Effect of Various Sterilization Procedures for in vitro Propagation of Carnation (Dianthus caryophyllus L.).Int.J.Curr.Microbiol.App.Sci. 8(2): 481-485. doi: https://doi.org/10.20546/ijcmas.2019.802.054