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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
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Int.J.Curr.Microbiol.App.Sci.2019.8(2): 10-15
DOI: https://doi.org/10.20546/ijcmas.2019.802.003


Evaluation of Two Rapid Field Level Diagnostic Tools for Acute Canine Leptospirosis in an Endemic Area
R. Ambily1*, M. Mini1, Siju Joseph1 and S.V. Krishna2
1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy -680 651, India
2Department of Veterinary Microbiology, College of Veterinary Science, Mamnoor Warangal Dist., India
*Corresponding author
Abstract:

The present study aims at evaluating the efficacy of two rapid tools, IgM dot ELISA and Latex Agglutination Test employing recombinant LipL32 as antigen in diagnosing acute leptospirosis in dogs in Kerala, a disease endemic area. One hundred and five serum samples were collected from dogs presented at Veterinary Hospitals attached to Kerala Veterinary and Animal Sciences University during the period from September 2014 to May 2016. Microscopic Agglutination test was carried out using the reference strains maintained in the Dept. of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy. In MAT, samples with titre ≥ 1:800 were considered as positive. For recombinant LipL32 protein production, the lipl32 gene of Leptospira interrogans was ligated with prokaryotic expression vector pPro.EX.HtC and transformed into competent E. coli DH5α cells. The clones were induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG) and the protein was purified using Nickel affinity chromatography and used as antigen in IgM dot ELISA, plate ELISA and Latex Agglutination Test (LAT). All the 105 samples were subjected to the three tests. The relative sensitivity and specificity of IgM dot ELISA, plate ELISA and LAT were evaluated in comparison with MAT. Among the 105 samples, 56 were found to have a titre of ≥1:800 in MAT. In IgM dot ELISA, 55 samples were found to be positive and one was negative. Latex Agglutination Test detected 58 samples as positive. It was found to be a less specific (91.84 %) test than IgM dot ELISA in detecting acute leptospirosis although it was found to be sensitive (96.43 %). IgM dot ELISA was proved to be sensitive (96.43 %) and specific (97.96 %) and the results were more satisfactory than IgM plate ELISA in detecting acute cases of leptospirosis which is very relevant in an endemic area like Kerala.


Keywords: Acute canine leptospirosis, pPro.EX.HtC, IgM dot ELISA, IgM plate ELISA
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How to cite this article:

Ambily, R., M. Mini, Siju Joseph and Krishna, S.V. 2019. Evaluation of Two Rapid Field Level Diagnostic Tools for Acute Canine Leptospirosis in an Endemic Area.Int.J.Curr.Microbiol.App.Sci. 8(2): 10-15. doi: https://doi.org/10.20546/ijcmas.2019.802.003