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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 3169-3183
DOI: https://doi.org/10.20546/ijcmas.2019.801.339


Bioprospecting of antibiofilm potential of Piper nigrum, Piper betle and Coscinium fenestratum on Escherichia coli
R. Ramya and S. M. Gopinath*
Research Center, Department of Biotechnology, Acharya Institute of Technology, Bengaluru, Karnataka, India- 560 107
*Corresponding author
Abstract:

Genetic language for social behavior in bacteria is mediated through Quorum sensing. This collective group behavior posses obvious advantages for the bacteria to adapt to suitable growth modes by migrating to amicable environmental conditions which makes them highly virulent and detrimental to the environment. 80% of the uncomplicated UTIs that occur in healthy individuals are due to uropathogenic E. coli which are the leading cause of morbidity. Current study investigates antibiofilm activity of methanol extracts from Coscinium fenestratum, Piper betle and Piper nigrum against clinically isolated, MDR E. coli. LC / MS-MS screened the herbal bioactive compounds. The extracts exhibited a MIC in a range of 0.1mg/mL- 0.15mg/mL. Reduction of EPS and rhamnolipids was found to be 79%, 68% and 62.0% respectively by C. fenestratum, P. betle and P. nigrum. Berberine and Eugenol can be used as quorum inhibitors for attenuation of MDR E. coli at sub inhibitory concentration of 50µg/mL.


Keywords: MDR, Biofilm, Quorum quenching, Piperidine, Eugenol, Berberine, E. coli
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How to cite this article:

Ramya, R. and Gopinath, S. M. 2019. Bioprospecting of antibiofilm potential of Piper nigrum, Piper betle and Coscinium fenestratum on Escherichia coli.Int.J.Curr.Microbiol.App.Sci. 8(1): 3169-3183. doi: https://doi.org/10.20546/ijcmas.2019.801.339