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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 95-105
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.011


Prevalence of Drug Resistant Gram Negative Bacilli (DRGNB) in Patients Suffering from Symptomatic Urinary Tract Infection from a Tertiary Care Hospital in Coimbatore, India
R.B. Varshini Narmada, R. Someshwaran* and Anbu N. Aravazhi
Department of Microbiology, Karpagam Faculty of Medical Sciences & Research, Othakalmandapam, Coimbatore-641032, India
*Corresponding author
Abstract:

Gram negative bacteria are highly adaptive pathogens that can develop resistance and evolve as Drug Resistant Gram Negative Bacilli (DR-GNB) by exhibiting Extended Spectrum Beta Lactamase (ESBLs), Amp C, NDM-1, Mcr-1 and Carbapenemase enzymes through several mechanisms. The main aim of this research was to study the prevalence of drug resistant gram negative bacilli in patients suffering from symptomatic urinary tract infections. After obtaining the IHEC clearance and informed consent form, nearly 820 urine samples were included in the study. Culture and sensitivity performed and various drug resistance patterns such as ESBLs, Amp C, K1 Enzymes, Carbapenemase production were detected and confirmed using various screening and confirmation tests. A total of 820 mid stream urine samples were collected from the subjects during the two months period. The mean age group of the study population was found to be 44.2 ± 0.2 years. Out of the 820 urine samples processed for Culture and sensitivity, nearly 166 samples (20.2%) grew bacteria and were termed ‘Culture positives’ and 654 samples (79.7%) were sterile and were termed ‘Culture negatives’. The incidence of Urinary tract infection was 20%. ESBL production was observed in 17 out of 140 isolates (12.14%). The antimicrobial resistance pattern is highly variable and a continuous surveillance of trends in resistance pattern is a borne necessity. To conclude Escherichia coli is the predominant pathogen isolated. The Prevalence of Drug resistant Gram Negative Bacilli thrusts a major therapeutic concern for the clinicians. Molecular method like Polymerase Chain Reaction is the Gold standard for confirmative diagnosis of these drug resistant bugs.


Keywords: Antimicrobial chemotherapy - Antibiotic policy - Antibiotic Resistance - Culture and Sensitivity - Infection control - Urinary isolates.
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How to cite this article:

Varshini Narmada, R.B., R. Someshwaran and Anbu N. Aravazhi. 2016. Prevalence of Drug Resistant Gram Negative Bacilli (DRGNB) in Patients Suffering from Symptomatic Urinary Tract Infection from a Tertiary Care Hospital in Coimbatore, India.Int.J.Curr.Microbiol.App.Sci. 5(12): 95-105. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.011