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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2973-2979
DOI: https://doi.org/10.20546/ijcmas.2019.801.316


Molecular Characterization of Brucella melitensis Detected from Aborted Sheep
Vinay Kumar, Aman Kumar, Sushila Maan, Nitish Bansal and Trilok Nanda
Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004, India
*Corresponding author
Abstract:

Brucellosis is one of the world’s major zoonoses that affect wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis, the disease is still endemic among cattle, buffaloes, sheep and goats. In many developing countries including India, Brucella melitensis is of much concern due to heavy economic losses to animal husbandry sector as well as humans. The present study was carried out to detect and characterize B. melitensis from biological samples (blood, vaginal swabs and aborted foetal material from 8 animals) collected from a disease outbreak in sheep flock using TaqMan probe based real time PCR (qPCR) assay and nucleotide sequencing. Out of 20 biological samples, 6 (30%) were found positive for Brucella spp. specific and Brucella melitensis specific qPCR assay. Further B. melitensis was confirmed by sequencing of the 1343 bp of 16S rRNA gene generated in conventional PCR.


Keywords: Brucella melitensis, Sheep, Real time PCR, Sequencing
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How to cite this article:

Vinay Kumar, Aman Kumar, Sushila Maan, Nitish Bansal and Trilok Nanda. 2018. Molecular Characterization of Brucella melitensis Detected from Aborted Sheep.Int.J.Curr.Microbiol.App.Sci. 8(1): 2973-2979. doi: https://doi.org/10.20546/ijcmas.2019.801.316