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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2926-2935
DOI: https://doi.org/10.20546/ijcmas.2019.801.311


Distribution of Oligomannosyl Side Chains in the Cell Wall Mannan of Pichia pastoris Purified by Benanomicin A
Takuya Kuraoka1, Momoka Shukuri1, Saki Iwanaga1, Takayoshi Yamada2, Yukiko Ogawa1 and Hidemitsu Kobayashi1*
1Laboratory of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan
2Sanko Medical Tomato Pharmacy Co., Ltd., 2023-2, Shimogumigo, Higashisonogigun, Kawatanacho, Nagasaki, 859-3615, Japan
*Corresponding author
Abstract:

In the previous study, a new method was established to prepare mannan under mild conditions by using antibiotic Benanomicin A. In this study, this method was applied to the isolation of P. pastoris NBRC 0984 mannan which predominantly contains β-1,2-linked mannose residues. As the findings of nuclear magnetic resonance (NMR) analysis of the resultant mannan to examine the distribution state of the side chain, it was found that despite the existence of oligomannosyl side chains corresponding to pentaose, Manα1→2Manβ1→Manβ1→Manα1→2Man, tetraose, Manβ1→Manβ1→Manα1→2Man, and biose, Manα1→2Man, in this molecule, the side chain corresponding to triose, Manβ1→Manα1→2Man, was not detected at all. In our previous study, a relatively large number of biosyl and triosyl side chains were detected in analyzes applying acetolysis to mannans prepared by the Fehling method from the same yeast cells. Such a difference can be explained as that the β-1,2-linkages of some pentaosyl and tetraosyl side chains were cleaved by acetolysis, and triose and biose occurred secondarily in large quantities. In conclusion, the best way to accurately measure the side chain distribution of mannan is to perform NMR analysis on untreated mannan molecules prepared under mild conditions using Benanomicin A.


Keywords: Pichia pastoris, Cell wall mannan, Benanomicin A, Nuclear Magnetic Resonance (NMR)
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How to cite this article:

Takuya Kuraoka, Momoka Shukuri, Saki Iwanaga, Takayoshi Yamada, Yukiko Ogawa and Hidemitsu Kobayashi. 2018. Distribution of Oligomannosyl Side Chains in the Cell Wall Mannan of Pichia pastoris Purified by BenanomicinInt.J.Curr.Microbiol.App.Sci. 8(1): 2926-2935. doi: https://doi.org/10.20546/ijcmas.2019.801.311