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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2870-2875
DOI: https://doi.org/10.20546/ijcmas.2019.801.302


Genetic Diversity within the Genus Pleurotus Determined by RAPD Analysis
Ravi Prakash Mishra, Manjul Pandey*, U.K. Tripathi and M. Singh
Department of Plant Pathology, C.S. Azad University of Agriculture and Technology, Kanpur-208002, India
*Corresponding author
Abstract:

Random amplified polymorphism DNA (RAPD) analysis was done to assess the diversity among ten species of Pleurotus. Understanding the pattern of fundamental not only addressing questions concerning evolutionary process and the development of conservation strategies, but also a prerequisite of the efficient use of genetic resources in breeding programme. The RAPD dendogram obtained by using a UPGMA programme grouped in to the investigated strains in to 5 clusters. RAPD bands were scored as present (1) or absent (0) for all the Pleurotus isolate. Each band was assumed to represent a unique genetic locus. The pattern and extent of RAPD variation were analyzed with respect to primer, polymorphic locus and isolate. Total number of amplified fragment and polymorphic fragment produced by 40 decamer primer were 229 and 226, respectively. Polymorphism percentage was 98.69. Ten primer SBSA11, SBSA13, SBSA15, SBSA16, SBSA18, SBSA19, SBSA20, SBSB14, SBSB15 and SBSB17 were not amplified the DNA from any of the isolate.


Keywords: Genomic DNA polymorphism, RAPD, Pleurotus species, Clusters
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How to cite this article:

Ravi Prakash Mishra, Manjul Pandey, U.K. Tripathi and Singh, M. 2019. Genetic Diversity within the Genus Pleurotus Determined by RAPD Analysis.Int.J.Curr.Microbiol.App.Sci. 8(1): 2870-2875. doi: https://doi.org/10.20546/ijcmas.2019.801.302