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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2575-2583
DOI: https://doi.org/10.20546/ijcmas.2019.801.270


Characterization of Antinematicidal and Antifungal Bacterial Microbes by 16s Rrna Gene Sequence
P. Vetrivelkalai1*and M. Sivakumar2
1Department of Fruit Crops
2Department of Nematology, Tamil Nadu Agricultural University, Coimbatore- 641 003, Tamil Nadu, India
*Corresponding author
Abstract:

The culture filtrates of endophytic bacterial isolates viz., EB16, EB18, EB19and EB3 significantly inhibited the egg hatching (93.36, 93.72, 91.08 and 85.80 per cent respectively), causing the juvenile mortality (95.67, 89.0, 82.67 and 77.33 per cent respectively) of root knot nematode, Meloidogyne incognita at 100% concentrations with 60h of exposure. All the four isolates significantly inhibited the mycelial growth of fungal pathogens viz., Fusarium oxysporum f.sp. lycopersici and Rhizoctonia solani in vitro. The four promising endophytic bacterial isolates viz., EB16, EB18, EB1 and 9 EB3 were identified as Bacillus cereus (Accession no. GU 321330), Bacillus pumilus (Accession no. GU 321331), Methylobacterium radiotolerans and Brevundimonas diminuta (Accession no. GU 321330), respectively by 16S rRNA gene sequence and phylogenetic tree construction.


Keywords: Endophytic bacteria, 16S rRNA gene, Antinematicidal, Antifungal and In vitro
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How to cite this article:

Vetrivelkalai, P. and Sivakumar, M. 2019. Characterization of Antinematicidal and Antifungal Bacterial Microbes by 16s Rrna Gene Sequence.Int.J.Curr.Microbiol.App.Sci. 8(1): 2575-2583. doi: https://doi.org/10.20546/ijcmas.2019.801.270