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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2517-2542
DOI: https://doi.org/10.20546/ijcmas.2019.801.266


Characterization of Thermally Stable β Galactosidase from Anoxybacillus flavithermus and Bacillus licheniformis Isolated from Tattapani Hotspring of North Western Himalayas, India
Varsha Rani*, Parul Sharma and Kamal Dev
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh, India
*Corresponding author
Abstract:

Nineteen thermophilic bacterial isolates were screened and only two (PW10 and PS7) produced extracellular, auto inducible β-galactosidase. PW10 and PS7 was Gram’s positive, rod shaped and exhibit growth between 50-80 °C and pH 5-9. Optimum β-galactosidase activity of 32083.33 U/mg/min was observed at 60 °C and pH 7 for PS7, while 2666.66 U/mg/min at 60 °C and pH 9 for PW10. 16S rDNA sequencing of PW10 showed 99% similarity with Anoxybacillus flavithermus and PS7 with Bacillus licheniformis (GenBank accession no. KF039883 and KF039882). Lactose supplementation enhanced β-galactosidase production by 7.6 folds in PS7, while 2.5 folds in PW10. Ethanol and hydrogen peroxide does not affect growth of PS7 isolate, while ethanol decreased the growth by 7.3 folds. Hydrogen peroxide inhibited growth of PW10. β-galactosidase of PS7 was metal independent, while β-galactosidase was metal activated in PS10. Presence of lactose and glucose activated β-galactosidase, while glucose did not affect -galactosidase activity in both isolates. Maximum β-galactosidase production was observed at ~ 72 h of incubation. Km value of 8.0 mM with ONPG (60° C) was determined for PS7 and 1.3 mM for PW10. β-galactosidase of both isolates was stable at 4 and 25 °C for 5-6 days.


Keywords: Thermophilic β-galactosidase, Lactose intolerance, Galactooligo saccharides, Prebiotic, Thermostable
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How to cite this article:

Varsha Rani, Parul Sharma and Kamal Dev. 2019. Characterization of Thermally Stable β galactosidase from Anoxybacillus flavithermus and Bacillus licheniformis Isolated from Tattapani Hotspring of North Western Himalayas, India.Int.J.Curr.Microbiol.App.Sci. 8(1): 2517-2542. doi: https://doi.org/10.20546/ijcmas.2019.801.266