National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Twenty five biodegradable waste samples were collected from different waste dumping sites of district Baramulla from five locations viz., Wadura, Sopore, Baramulla town, Bomai and Pattan. The cellulolytic bacteria were isolated on Carboxyl Methyl Cellulose Agar Medium (CMC) by following serial dilution pour plate method. The cellulolytic bacterial isolates were screened qualitatively as well as quantitatively at three different temperatures (10, 15 & 20oC) and three different pHs (5, 7 and 9). Out of twenty, CBW3 isolate from Wadura showed highest solubilization zone (4 mm) with solubilization efficiency (285.7%) and cellulase activity (2.917 U/ml) followed by CBB3 from Baramulla town (3.4 mm) with solubilization efficiency (261.5%) and cellulase activity (2.566 U/ml). All the 20 cellulolytic bacterial isolates were morphologically, biochemically characterized. Although these microbes showed the cellulose activity under variable pH and temperature combinations but pH 7 and 20 oC temperature was the most ideal standardized condition for the better performance by the microbes under investigation.
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