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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2033-2048
DOI: https://doi.org/10.20546/ijcmas.2019.801.213


Characterization of Cellulolytic Bacteria from Waste Dumping Sites of Kashmir Himalaya
Bisma Rashid1*, Zahoor Ahmad Baba2, Misbah Ajaz Malik1, Aamir H. Mir2, Farida Akhter2, Malik Asif1, M.Y. Zargar1, Nighat Rashid3, Nowsheen Rashid3 and Showkat Maqbool4
1Division of Basic Science and Humanities
2Division of Soil Science and Agricultural Chemistry
3Department of Management Studies
4Division of Agricultural Statistics, Sher e Kashmir University of Agricultural Sciences and Technology of Kashmir-193201, University of Kashmir-190006, India
*Corresponding author
Abstract:

Twenty five biodegradable waste samples were collected from different waste dumping sites of district Baramulla from five locations viz., Wadura, Sopore, Baramulla town, Bomai and Pattan. The cellulolytic bacteria were isolated on Carboxyl Methyl Cellulose Agar Medium (CMC) by following serial dilution pour plate method. The cellulolytic bacterial isolates were screened qualitatively as well as quantitatively at three different temperatures (10, 15 & 20oC) and three different pHs (5, 7 and 9). Out of twenty, CBW3 isolate from Wadura showed highest solubilization zone (4 mm) with solubilization efficiency (285.7%) and cellulase activity (2.917 U/ml) followed by CBB3 from Baramulla town (3.4 mm) with solubilization efficiency (261.5%) and cellulase activity (2.566 U/ml). All the 20 cellulolytic bacterial isolates were morphologically, biochemically characterized. Although these microbes showed the cellulose activity under variable pH and temperature combinations but pH 7 and 20 oC temperature was the most ideal standardized condition for the better performance by the microbes under investigation.


Keywords: BW3, CBB3, Screening, Solubilization zone, Cellulase enzyme, Kashmir
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How to cite this article:

Bisma Rashid, Zahoor Ahmad Baba, Misbah Ajaz Malik, Aamir H. Mir, Farida Akhter, Malik Asif, M.Y. Zargar, Nighat Rashid, Nowsheen Rashid and Showkat Maqbool. 2019. Characterization of Cellulolytic Bacteria from Waste Dumping Sites of Kashmir Himalaya.Int.J.Curr.Microbiol.App.Sci. 8(1): 2033-2048. doi: https://doi.org/10.20546/ijcmas.2019.801.213