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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 898-905
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.103


Factorial Design based Medium Optimization for the Improved Production of Biosurfactant by Mucor polymorphosphorus
Patrícia Mendes Souza1,4, Marta Freitas-Silva1,4, Thayse Alves de Lima e Silva1,4, Grayce K.B. Silva1,4, Marcos A.B. Lima2,4, Aline Elesbão Nascimento4, Nathália Marques3,4, Vanessa Pimentel Santos3,4, Laura Truan Oliveira3,4 and Galba M.Campos-Takaki4*

1National Program of Post-Doctorate (PNPD) –CAPES, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil;

2Department of Biology, Federal Rural University of Pernambuco, 52.171-900

Recife, Pernambuco, Brazil;

3Master  in Development of Environmental Processes , Catholic University of Pernambuco, 50.050-900 Recife, Pernambuco, Brazil.

4Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil
*Corresponding author
Abstract:

The biosurfactants are compounds of microbial origin that exhibit surfactant property, low toxicity and high biodegradability. In this study was to evaluate the production of surfactant by Mucor polymorphosphorus, isolated from Caatinga soil from Brazil, using agro-industrial residues. The experiments were monitored by Central Composite Rotational Design (CCRD) using as variable response the reduction of the surface tension.  The factors selected for study were soybean waste oil and corn steep liquor as substrates. The spore suspension containing 107sporangiospores/mL were inoculated in Erlenmeyer flasks containing the alternative medium according factorial design; The flasks were grown during 96 hours, at 28°C and 150 rpm. All factors studied were important within the ranges investigated. The empirical forecast model developed regarding effective nutritional factors was adequate for explain 84.79% of the variation observed on biosurfactant production. Maximal reduction in surface tension of 33.2 mN.m-1 with final pH 6.5 was obtained under the optimal conditions of corn steep liquor 4.61% and soybean oil after frying 7.5%. These results demonstrated that the factorial design is adequate for identifying the optimal conditions for biosurfactant production by fungi. 


Keywords: Optimization; Factorial Desing; Mucor polymorphosphorus; Tensio-active agents; Agro-industrial residues; Biosurfactant
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How to cite this article:

Patrícia Mendes Souza, Marta Freitas-Silva, Thayse Alves de Lima e Silva, Grayce K.B. Silva, Marcos A.B. Lima, Aline Elesbão Nascimento, Nathália Marques, Vanessa Pimentel Santos, Laura Truan Oliveira and Galba M.Campos-Takaki. 2016. Factorial Design based Medium Optimization for the Improved Production of Biosurfactant by Mucor polymorphosphorus. Int.J.Curr.Microbiol.App.Sci. 5(11): 898-905. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.103