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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 1714-1721
DOI: https://doi.org/10.20546/ijcmas.2019.801.182


Morphological and Molecular Characterization of Rhizoctonia solani causing Sheath Blight in Rice
Suryawanshi Padmaja Pralhad1*, P.U. Krishnaraj2 and S.K. Prashanthi3
1Department of Biotechnology,
2Department of Agricultural Microbiology
3Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Dharwad - 580005, Karnataka, India
*Corresponding author
Abstract:

Sheath blight of rice is an economically important pathogen of rice worldwide. The simple methods based on morphological markers can be used to identify the associated pathogens. In the present study, three fungal isolates were studied for morphological and pathological characters. They were fast growing in culture medium with differences in sclerotia formation and exhibited varying degree of virulence on the same cultivar BPT5204, a variety susceptible to sheath blight. The isolate RS4 was found to be highly virulent with 78% disease incidence. Precise identification of cause of disease based on morphological characters and symptoms induced by Rhizoctonia sp. becomes tedious because of similarity in symptoms. The identification of isolates at genus and species level using molecular markers for genetic differentiation would be an ideal approach. The isolate RS4 showed 99 % homology with R. solani AG1-IA based on nucleotide sequence data for ITS 5.8S-rDNA region.


Keywords: Sheath blight, Rice, Rhizoctonia solani, Sclerotium, Pathogen, Virulence
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How to cite this article:

Suryawanshi Padmaja Pralhad, P.U. Krishnaraj and Prashanthi, S.K. 2019. Morphological and Molecular Characterization of Rhizoctonia solani causing Sheath Blight in Rice.Int.J.Curr.Microbiol.App.Sci. 8(1): 1714-1721. doi: https://doi.org/10.20546/ijcmas.2019.801.182