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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 1556-1563
DOI: https://doi.org/10.20546/ijcmas.2019.801.163


Isolation, Screening and Characterization of Chitinase Producing Fungi from Apple Orchards of Shimla and Kinnaur District, India
Nirja Thakur1*, Rakesh Gupta2, Amarjit K. Nath1, Anjali Chauhan3, Manisha Thakur1, R.K. Dogra4 and Himanshu Pandey1
1Department of Biotechnology
2College of Horticulture
3Department of Soil Science & Water Management
4Department of Fruit Science, Dr Y S Parmar, UHF, Nauni, Solan (H.P.), India
*Corresponding author
Abstract:

Aims of present study were to isolate and characterize chitinase producing fungi from soil samples of apple orchards of Shimla and Kinnaur district of Himachal Pradesh. The soil samples were collected aseptically and subjected to serial dilution to isolate the fungal strains. Total nine morphologically different fungi were isolated and screened for their chitinolytic activity in colloidal chitin incorporated media through zone assay. The isolates were screened based on the size of the zone formed. Best chitinase producers were subjected 18S ribosomal RNA sequencing. After molecular characterization of two isolates, they were identified as Alternaria brassicicola strain and Pencillium sp. isolate. A novel strain, Acinetobacter ASK18, a gram-negative, motile organism was identified. These isolates would further be subjected to purification of the enzyme produced and hence could be evaluated as effective biocontrol agents against pathogenic bacteria and fungi.


Keywords: Soil, Chitinase, Colloidal chitin, 18S rRNA sequencing
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How to cite this article:

Nirja Thakur, Rakesh Gupta, Amarjit K. Nath, Anjali Chauhan, Manisha Thakur, R.K. Dogra and Himanshu Pandey. 2019. Isolation, Screening and Characterization of Chitinase Producing Fungi from Apple Orchards of Shimla and Kinnaur District, India.Int.J.Curr.Microbiol.App.Sci. 8(1): 1556-1563. doi: https://doi.org/10.20546/ijcmas.2019.801.163