National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A field experiment was conducted with efficient isolates of endophytic nitrogen fixing bacteria i.e., Gluconacetobacter diazotrophicus NFGS25 and NFGM5 which were isolated from the roots of sugarcane and maize, based on the results of pot culture studies on maize. NFGS25 and NFGM5 were confirmed by the molecular characterization using 16S rDNA method. At 30 DAS(days after sowing), the growth parameters such as plant height (52.17 cm), stem girth (3.53 cm), chlorophyll content (32.5), root length (60 cm), dry matter production of shoot (3.5 g per plant) and root (0.57 g per plant) of maize were maximum in the treatment inoculated with NFGM5 at 75 % RDN level. The per cent shoot nitrogen content was maximum in the treatment inoculated with, NFGM5 and NFGS25 at 75 % RDN (0.37 %). At 100 % RDN, NFGM5 recorded higher shoot nitrogen of 0.27 % as compared to NFGS25 (0.23 %) at 30 DAS. The nitrogen uptake was significantly higher in the treatment NFGM5 at 75 %RDN (8.65 g plant-1) followed by NFGS25 (7.73 g plant-1). The population of G. diazotrophicus was maximum in the treatment inoculated with NFGM5 at 75 % RDN (9.8 x 104 CFU g-1) followed by NFGS25 (3.5 x 104 CFU g-1) at 60 DAS. The yield parameters like number of cobs per plant (2.07), cob weight per plant (295.93 g), grain yield per plant (250.03 g), grain yield (183.33 q ha-1) and test weight (40.17 g) were significantly highest in the treatment NFGM5 at 75 % RDN followed by NFGS25 at 75 % RDN. It is evident that, Gluconacetobacter diazotrophicus isolates inoculated with graded level of recommended dose of nitrogen promotes plant growth and grain yield under field condition.
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