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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 1393-1403
DOI: https://doi.org/10.20546/ijcmas.2019.801.148


Effect of VAM on ex vitro Hardening of Doubled Haploid Line of Marigold (Tagetes erecta L.) Variety Local Orange
Uzma Mehraj1*, Sapna Panwar1, Kanwar Pal Singh1, Namita1 and Seema Sagwan2
1Division of Floriculture and Landscaping
2Division of Microbiology, ICAR- Indian Agricultural Research Institute, New Delhi- 110012, India
*Corresponding author
Abstract:

In this study, the effect of Vesicular Arbrbusular Mycorrhizae (VAM) on the ex vitro hardening of marigold was investigated. A protocol was generated for ex vitro hardening of in vitro raised plantlets using VAM consortia (Glomus intraradices, Glomus mosseae and Scutellospora spp.) in different media mixtures, and their effect was observed on plant survival and growth. Highest percent survival (94.00), maximum plant height (39.00 cm), maximum plant spread (30.50 cm), maximum stem diameter (0.97 cm), maximum shoot fresh weight (0.75 g), maximum root fresh weight (0.46 g), maximum leaf length (10.27 cm), maximum root length (28.50 cm) and maximum leaf width (6.82 cm) were observed in treatment comprising of Soil + Vermicompost (3:1) + 20 g VAM consortia, Followed by 92.00% plant survival, 36.75 cm plant height, 29.00 cm plant spread, 0.81 cm stem diameter, 0.58 g shoot fresh weight, 0.45 g root fresh weight, 10.20 cm leaf length, 20.88 cm root length, and 6.76 cm leaf width in treatment comprising Soil + Vermicompost (1:1) + 20 g VAM consortia. While minimum percent survival (72.50%), lowest height (26.00 cm), minimum plant spread (9.50 cm), minimum stem diameter (0.49 cm), minimum shoot fresh weight (0.27 g), minimum root fresh weight (0.14 g), minimum leaf length (8.06 cm), minimum root length (10.27 cm) and minimum leaf width (3.86 cm) were observed in control media comprising of only soil. Followed by 85.00% survival, 29.25 cm plant height, 17.75 cm plant spread, 0.51 cm stem diameter, 0.34 g shoot fresh weight, 0.28g root fresh weight, 8.07 cm leaf length, 14.05 cm root length and 4.08 cm leaf width were observed in media comprising of Soil + FYM (1:1) + 10 g VAM consortia. Highest percent colonization (60.78) was showed in media comprising of Soil + Vermicompost (3:1) + 20 g VAM consortia, followed by 57.65 % in media comprising of Soil + Vermicompost (1:1) + 20 g VAM consortia. While lowest root colonization of 47.28% was found in media comprising of Soil + FYM (1:1) + 10 g VAM consortia followed by 50.37% in media comprising of Soil+ FYM (3:1) +10 g VAM consortia. Control treatment, without any VAM added, showed no root colonization. Hence, it was concluded that the treatment with a mixture of Soil + Vermicompost (3:1) with 20 g VAM consortia showed the best results in various aspects.


Keywords: Tagetes erecta L., Doubled haploid line, Ex vitro hardening, VAM
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How to cite this article:

Uzma Mehraj, Sapna Panwar, Kanwar Pal Singh, Namita and Seema Sagwan. 2019. Effect of VAM on ex vitro Hardening of Doubled Haploid Line of Marigold (Tagetes erecta L.) Variety Local Orange.Int.J.Curr.Microbiol.App.Sci. 8(1): 1393-1403. doi: https://doi.org/10.20546/ijcmas.2019.801.148