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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 357-370
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.041


Phylogenetic Diversity of Bacterial Communities of Western India Oil Fields by PCR-DGGE
Mahesh Chitrakoti1* and Kalpana Chandramore2
1 2Vidya Pratishthan’s Arts, Science and Commerce College, Baramati, India
*Corresponding author
Abstract:

The diversity of bacterial communities in the formation waters of ten oil reservoirs from western India was analyzed by PCR – Denaturing Gradient Gel Electrophoresis (DGGE). 16S rRNA gene fragments of the bacterial community were PCR amplified using universal primers targeting V3-V5 eubacterial hyper-variable regions. The phylogenetic analysis revealed that formation water metagenome mainly constituted bacteria, showing similarity to Paracoccus, Brachymonas, Hydrogenophaga, Bacillus, Pelomonas, Thalasospira and Pseudomonas. Many of the bacterial sequences shared less than 95% homology with the reference sequences available in Gen-Bank database, hence; some of these could represent novel species/ genera. Application of statistical indices such as Simpson's index (1- D) and the Shannon-Weaver index (H) to the well resolved DGGE pattern of 16S rR NA gene fragments revealed more diversity of bacteria in S7 and S9 samples, whereas equitability (J) data indicated that population was not dominated by any species. It was also observed that formation water consisted of predominantly the proteobacteria capable of nitrate reduction and hydrocarbon degradation.


Keywords: Bacterial community, DGGE, Indian oil reservoirs, Phylogenetic analysis.
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How to cite this article:

Mahesh Chitrakoti and Kalpana Chandramore. 2016. Phylogenetic Diversity of Bacterial Communities of Western India Oil Fields by PCR-DGGE. Int.J.Curr.Microbiol.App.Sci. 5(11): 357-370. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.041