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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 1172-1182
DOI: https://doi.org/10.20546/ijcmas.2019.801.123


Rapid Identification of Mycobacterium tuberculosis and Non Tuberculous Mycobacterium Isolates from Pulmonary and Extra Pulmonary Samples using MGIT320 Liquid Culture System and MPT64 Antigen Test
Qursheed Sultana, Ajaz Hussain*, Mohammed Abdur Rab Ansari, Mohd Khaleel and Maimoona Mustafa
Department of Microbiology, Deccan College of Medical Sciences, Hyderabad, India
*Corresponding author
Abstract:

Tuberculosis (TB) is a major public health problem in India and a leading cause of death in adults, especially among the economically productive age group. Historically TB has been associated with significant morbidity and mortality and remains a major global health problem. The present study was initiated to determine the prevalence of Mycobacterium tuberculosis, Non Tuberculous Mycobacterium and its resistance to first line Anti-Tubercular drug from both pulmonary and extra pulmonary samples A total of 583 properly collected samples (226 pulmonary and 357 extra pulmonary) from patients with clinical/radiological suspicion of Tubercular infection were included in this study. All the samples were screened by Zeihl-Neelsen AFB microscopy, and subjected to liquid culture using Mycobacterium Growth Indicator Tube (MGIT-320). Positive cultures were differentiated into Mycobacterium tuberculosis complex (MTBc) or non-tubercular mycobacterium (NTM) by immunochromatography assay using MPT-64 antigen. Further it was followed by drug susceptibility testing of MTBc isolates thereby identifying multi-drug resistant strains. Out of 583 samples, 141 strains were isolated on MGIT-320 (81 pulmonary, 60 Extrapulmonary) and the detection time was 15 days. Mycobacterium complex isolates were 116 and Nontuberculous Mycobacteria were 25. Among Mycobacterium tuberculosis complex isolates 92(56 pulmonary, 36 Extrapulmonary) were sensitive to all the drugs and 24(16 pulmonary, 8 Extrapulmonary) were resistant to one or more drugs. Multiple drug resistant (MDR) isolates were 7(6 pulmonary, 1 Extrapulmonary). MDR-TB is gradually increasing due to improper diagnosis and inadequate treatment. Differentiating mycobacterium as MTBc and NTM supported by sensitivity testing by using liquid culture has proved to be helpful in early decision for chemotherapy in MDR-TB patients.


Keywords: Mycobacterial growth indicator tube (MGIT), Mycobacterium tuberculosis complex (MTBc), Non-tubercular mycobacterium (NTM), MPT64 antigen test and multiple drug resistant (MDR)
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How to cite this article:

Qursheed Sultana, Ajaz Hussain, Mohammed Abdur Rab Ansari, Mohd Khaleel and Maimoona Mustafa. 2019. Rapid Identification of Mycobacterium tuberculosis and Non Tuberculous Mycobacterium Isolates from Pulmonary and Extra Pulmonary Samples using MGIT320 Liquid Culture System and MPT64 Antigen Test.Int.J.Curr.Microbiol.App.Sci. 8(1): 1172-1182. doi: https://doi.org/10.20546/ijcmas.2019.801.123