National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The Artocarpus heterophyllus Lam commonly known as jackfruit belongs to the family Moraceae. The Western Ghats of India is believed to be the centre of origin of jackfruit. A study on Molecular characterization and DNA fingerprinting of promising selections of jackfruit (Artocarpus heterophyllus Lam.) using SSR markers was carried out at Regional Agricultural Research Station (R.A.R.S.), Kumarakom and Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara, Kerala during the period 2015-2017. The objectives of the study were to characterize jackfruit varieties using SSR markers and to develop DNA fingerprint with which the variety could be identified and its fidelity is detected. A total of eight jack genotypes including one KAU released jack variety (Sindhur), one Cultivar (Muttom varikka) and six superior jackfruit selections (Veloor varikka-1, Veloor varikka-2, Kavanar varikka-1, Pathamuttom varikka-1, Pathamuttom Varikka-2 and Chengalam varikka) identified at RARS, Kumarakom were utilized for the study. DNA extraction was done with CTAB method with slight modification. A set of 50 SSR primers reported from the related genera were screened for polymorphism. The PCR products obtained from SSR analysis were separated on 3 percent high resolution agarose gel and the amplification patterns were observed. Eleven SSR primers which showed maximum polymorphism were selected for fingerprinting. The amplification pattern obtained with these primers were scored and depicted to develop fingerprint for each variety. Most of the amplicons were found to be shared among the genotypes. However, the pattern of sharing was different and good enough to separate out most of the varieties. Unique amplicons were observed for Sindhur, Pathamuttom varikka-1, Pathamuttom varikka-2 and Chengalam varikka, which can act as specific fingerprint of these genotypes. Among the SSR primers, MAA145 showed 100% polymorphism. The PIC value for SSR primers ranged from 0.22 to 0.98 with an average of 0.79 and MI value varied from 0.66 to 2.7 with an average of 1.18.
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