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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Int.J.Curr.Microbiol.App.Sci.2019.8(1): 679-688
DOI: https://doi.org/10.20546/ijcmas.2019.801.076


Molecular Characterization and DNA Fingerprinting of Superior Jackfruit Genotypes from Kerala using SSR Markers
P.S. Marjan1, Anu G. Krishnan2* and Anu Cyriac2
1College of Horticulture, Vellanikkara, KAU P.O, Thrissur, Kerala, India
2Regional Agricultural Research Station, Kumarakom, Kottayam, Kerala, India
*Corresponding author
Abstract:

The Artocarpus heterophyllus Lam commonly known as jackfruit belongs to the family Moraceae. The Western Ghats of India is believed to be the centre of origin of jackfruit. A study on Molecular characterization and DNA fingerprinting of promising selections of jackfruit (Artocarpus heterophyllus Lam.) using SSR markers was carried out at Regional Agricultural Research Station (R.A.R.S.), Kumarakom and Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara, Kerala during the period 2015-2017. The objectives of the study were to characterize jackfruit varieties using SSR markers and to develop DNA fingerprint with which the variety could be identified and its fidelity is detected. A total of eight jack genotypes including one KAU released jack variety (Sindhur), one Cultivar (Muttom varikka) and six superior jackfruit selections (Veloor varikka-1, Veloor varikka-2, Kavanar varikka-1, Pathamuttom varikka-1, Pathamuttom Varikka-2 and Chengalam varikka) identified at RARS, Kumarakom were utilized for the study. DNA extraction was done with CTAB method with slight modification. A set of 50 SSR primers reported from the related genera were screened for polymorphism. The PCR products obtained from SSR analysis were separated on 3 percent high resolution agarose gel and the amplification patterns were observed. Eleven SSR primers which showed maximum polymorphism were selected for fingerprinting. The amplification pattern obtained with these primers were scored and depicted to develop fingerprint for each variety. Most of the amplicons were found to be shared among the genotypes. However, the pattern of sharing was different and good enough to separate out most of the varieties. Unique amplicons were observed for Sindhur, Pathamuttom varikka-1, Pathamuttom varikka-2 and Chengalam varikka, which can act as specific fingerprint of these genotypes. Among the SSR primers, MAA145 showed 100% polymorphism. The PIC value for SSR primers ranged from 0.22 to 0.98 with an average of 0.79 and MI value varied from 0.66 to 2.7 with an average of 1.18.


Keywords: Jackfruit, SSR markers, DNA fingerprinting, Genetic diversity, Dendrogram
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How to cite this article:

Marjan, P.S., Anu G. Krishnan and Anu Cyriac. 2019. Molecular Characterization and DNA Fingerprinting of Superior Jackfruit Genotypes from Kerala using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 8(1): 679-688. doi: https://doi.org/10.20546/ijcmas.2019.801.076