Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 585-590
DOI: https://doi.org/10.20546/ijcmas.2019.801.065


Molecular Characterization of Brucella melitensis Field Isolates by Bruce- Ladder Multiplex PCR
B.R. Sumathi1*, B.M. Veeregowda2, S.M. Byregowda1, D. Rathnamma2, Shrikrishna Isloor2, Rajeswari Shome3 and H.D. Narayanaswamy2
1Institute of Animal Health and Veterinary Biologicals, KVAFSU, Hebbal, Bengaluru-560024, Karnataka, India
2Department of Microbiology, Veterinary College, KVAFSU, Hebbal, Bengaluru-560024, Karnataka, India
3Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR- NIVEDI), Post Box No. 6450, Yelahanka, Bengaluru-560064, Karnataka, India
*Corresponding author
Abstract:

Brucellosis still remains an infectious, highly contagious and re-emerging endemic zoonosis especially in the Mediterranean and Middle-East regions of the world involving many countries including India where it constitutes occupational hazard (Shakerian et al., 2013 and Khamesipour et al., 2014). It also poses a serious threat to livestock economy by causing abortion, loss of offspring, infertility and reduction in milk yield. The prevalence of brucellosis in animal reservoirs is an evidence of its prevalence in human population and control of animal brucellosis is the key to its control in humans. Early diagnosis is essential to minimise the spread of the disease besides public health importance. In the present study molecular characterization of five Brucella melitensis isolates was carried out through Bruce-ladder multiplex PCR and compared with Brucella abortus, Brucella melitensis and Brucella suis vaccine and challenge strains. The amplification profile confirmed the isolates as Brucella melitensis and there was a significant difference among these field isolates with that of reference vaccine strains and the amplicons of all the field isolates were similar to amplicons of reference challenge strain, Brucella melitensis 16M.


Keywords: Brucellosis, Brucella melitensis, Bruce-ladder multiplex PCR and Amplification profile
Download this article as Download

How to cite this article:

Sumathi, B.R., B.M. Veeregowda, S.M. Byregowda, D. Rathnamma, Shrikrishna Isloor, Rajeswari Shome and Narayanaswamy, H.D. 2019. Molecular Characterization of Brucella melitensis Field Isolates by Bruce- Ladder Multiplex PCR.Int.J.Curr.Microbiol.App.Sci. 8(1): 585-590. doi: https://doi.org/10.20546/ijcmas.2019.801.065