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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 447-455
DOI: https://doi.org/10.20546/ijcmas.2019.801.047


Morphological Variation and Chitinase Production Ability of Trichoderma viride Mutants
M.P. Vyawahare*, S.T. Ingle and A.G. Gathe
Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (M.S.), India
*Corresponding author
Abstract:

In an attempt to develop superior Trichoderma viride isolates for improvement in chitinase Enzyme productivity, induction of mutants was applied. After application of different concentrations of Ethyl methane sulfonate (EMS) and Hydroxyl amine (HA) treatments on T.viride mutants were obtained. Trichoderma viride was determined by its physiological state so that change in genetical conditions could alter the antagonism. Hence, the genetic modification using mutagenesis offers the potential for producing improved bioprotection is likely to enhance their biocontrol. Trichoderma viride mutants grown up to six generations to check the stability on PDA and it was found variation in mutants compared to mother culture. These mutants were tested for their chitinase productivity. TvME-4 (T4), TvMH-9 (T9) and TvME-3 (T3) mutants proved to be the highest producer of chitinase enzyme, since it produced 0.64, 0.63 and 0.62 enzyme units respectively, greater than the mother culture.


Keywords: Morphological variation, Chitinase production, Trichoderma viride, Mutants
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How to cite this article:

Vyawahare, M.P., S.T. Ingle and Gathe, A.G. 2019. Morphological Variation and Chitinase Production Ability of Trichoderma viride Mutants.Int.J.Curr.Microbiol.App.Sci. 8(1): 447-455. doi: https://doi.org/10.20546/ijcmas.2019.801.047