Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 774-781
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.089


In Vivo Studies comparing the Fever Patterns using Escherichia coli and Pseudomonas aeruginosa as Pyrogens in Rabbits
Hissah Al-Turki1, Samirah Al-Ali1, Reem Al-Khaldi1, Muneerah Al-Rowaila1,and Lorina Badger-Emeka2*
1College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia
2Department of Biomedical Sciences, College of Medicine, King Faisal University,Al-Ahsa, Saudi Arabia
*Corresponding author
Abstract:

The pyrogenic effects of microbial products have taken great attention of researchers due to its usefulness in pharmaceutical industries for the monitoring of adverse effects of contaminated drugs, intravenous fluids and vaccines. Fever pattern caused by bacterial pyrogens could also help as a guide during empiric antibiotic treatment in this era of multi-antibiotic resistant bacteria. The present research study seeks to evaluate the fever pattern amongst bacterial strains of some species through pyrogenic effects in rabbits as well as comparing the fever patterns between the different bacteria species. Gram-negative bacteria, Escherichia coli (ATCC 25922), non-coded E. coli, Pseudomonas aeruginosa (ATCC 27853) and non-coded P. aeruginosa were used for the investigations. Each rabbit was intravenously inoculated via the ear vein with one strain of bacteria and temperature was monitored rectally at 15 minutes intervals post bacteria exposure. The obtained results showed that fever was ascertained in all the rabbits irrespective of the bacteria strain. The maximum difference from initial starting temperature of between, 0.6°C to 0.9°C was obtained.  The results indicate fluctuations in temperature readings and revealed a significant (P value< 0.05) difference in the means of the temperature between non-coded P. aeruginosa and coded E. coli. Also, some of the rabbits exhibited hyperthermia signs during the experiment irrespective of the initial rabbit starting temperature nor the bacteria strain or species. Fever pattern exhibited by the two different gram negative organisms are seen to be bacteria species specific.


Keywords: Fever pattern, coli, aeruginosa, pyrogen.
Download this article as Download

How to cite this article:

Hissah Al-Turki, Samirah Al-Ali, Reem Al-Khaldi, Muneerah Al-Rowaila and Lorina Badger-Emeka. 2016. In Vivo Studies comparing the Fever Patterns using Escherichia coli and Pseudomonas aeruginosa as Pyrogens in Rabbits.Int.J.Curr.Microbiol.App.Sci. 5(11): 774-781. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.089