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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 3664-3679
DOI: https://doi.org/10.20546/ijcmas.2018.712.415


Characterization of Some Enteroviruses in Irrigating Water of Rural and Urban Areas in Egypt and in Clinical Samples with the Assessment of Ethanolic Extract of Nigella sativa in Response to these Viruses
Waled Morsy El-Senousy1, Faten S. Bayoumi2*, Sara A. Mokhtar3 and Rana K. Abd El-Tawab3
1Department of Water Pollution Research, Environmental Research Division and Food Borne Viruses Group, Centre of Excellence for Advanced Science, National Research Centre, Giza, Egypt 2Department of Microbiology and Immunology, Faculty of Pharmacy, MSA, Department of Immunogenetics, Human Genetics and genome research Division, National Research Centre, Giza, Egypt 3College of Pharmacy, MSA University, Egypt
*Corresponding author
Abstract:

This study aimed to investigate the frequency of enteroviruses in irrigation water of Egyptian rural and urban areas and also studying the effect of ethanolic extract of Nigella sativa on these viruses in vitro. 23 samples were collected from irrigation water sites which irrigate freshly eaten crops on El-Giza and El-Menofya Governorates from March 2017 to May 2017; fourteen samples from five rural sites in El-Menofya which no sanitation systems serve these regions and nine samples from three urban sites in El-Giza served by sanitation systems were investigated. From these samples, Adenoviruses were the highly detected (39.1%) followed by rota viruses (26.1%) and then noroviruses (8.7%). Presence of sanitation systems may affect the frequency of enteric viruses in irrigation water. Rotaviruses were the most frequent in the diarrheal samples collected from the same areas of irrigation water samples followed by adenoviruses and finally noroviruses. Considerable reduction of genome copies and infectious units of both rotavirus Wa strain and adenovirus type 7 was observed by treatment with ethanolic extract of Nigella Sativa in vitro.


Keywords: Noroviruses, adenoviruses, Rota viruses irrigating water, Real time PCR, Nigella sativa, RT-PCR, Cell Culture RT-PCR
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How to cite this article:

Waled Morsy El-Senousy, Faten S. Bayoumi, Sara A. Mokhtar and Rana K. Abd El-Tawab. 2018. Characterization of Some Enteroviruses in Irrigating Water of Rural and Urban Areas in Egypt and in Clinical Samples with the Assessment of Ethanolic Extract of Nigella sativa in Response to These Viruses.Int.J.Curr.Microbiol.App.Sci. 7(12): 3664-3679. doi: https://doi.org/10.20546/ijcmas.2018.712.415