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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 3629-3635
DOI: https://doi.org/10.20546/ijcmas.2018.712.411


Metritis Associated Aerobic Bacteria in Crossbred Dairy Cows and its Multiple Drug Resistance in Organized Dairy Farm, Maharashtra, India
Mahendra Mohan Yadav* and S.S. Jadhav
Veterinary Science, RCSM College of Agriculture, Kolhapur, Maharashtra – 416 004, India
*Corresponding author
Abstract:

A clinical investigation was conducted with the objective to isolate and identify the uterine aerobic bacteria in post partum crossbred dairy cows suffering from metritis and antibiotic sensitivity testing using disc diffusion test. Fourteen post partum crossbred dairy cows found positive for clinical metritis at Research cum Development Project (RCDP) on Cattle, Mahatma Phule Krishi Vidyapeeth (MPKV), Rahuri Maharashtra State were investigated in the present study. The biochemical characterization results indicated that E. coli was isolated from 4 samples; while 3 each sample was found positive for Klebsiella spp. and Staphylococcus spp. From the remaining 4 metritic samples, no growth was observed either on MacConkey or blood agar. Antibiotic sensitivity pattern revealed that highest resistance was observed in Ampicillin and Amoxycillin (90.0% each), followed by Co-trimoxazole and Enrofloxacin (70.0% each), Tetracycline (50.0%), Ceftriaxone and Gentamicin (40.0% each). While all the isolates were found sensitive to Chloramphenicol and Ciprofloxacin antibiotics. Multiple drug resistance was observed in 7 isolates. Earlier research findings at RCDP on Cattle, MPKV, Rahuri revealed that Enrofloxacin was the drug of choice for treatment of E. coli associated Metritis. But present indicated the development of resistance of E. coli to Enrofloxacin antibiotic. The paradigm shift in resistance pattern may be due to consistent use of Enrofloxacin for treatment of Metritis resulting into development of resistant mutants.


Keywords: Metritis, Aerobic bacteria, Crossbred Dairy cows, Antibiotic sensitivity testing and multiple drug resistance
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How to cite this article:

Mahendra Mohan Yadav and Jadhav, S.S. 2018. Metritis Associated Aerobic Bacteria in Crossbred Dairy Cows and its Multiple Drug Resistance in Organized Dairy Farm, Maharashtra, India.Int.J.Curr.Microbiol.App.Sci. 7(12): 3629-3635. doi: https://doi.org/10.20546/ijcmas.2018.712.411