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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 3611-3620
DOI: https://doi.org/10.20546/ijcmas.2018.712.409


Report of Novel Entomopathogenic Nematode (Heterorhab ditidoides sp.) in Telangana state, India
Amit. U. Paschapur1*, K. Vijaya Lakshmi2, Khushbu Chauhan3, B. S. Sunanda2, Chetna Mathur3, Chaitra Bhat3, Vishal Somavanshi3, Uma Rao3 and Vinod Pawar2
1Division of Entomology
3Division of Nematology, ICAR-IARI, New Delhi- 110012, India
2National Institute of Plant Health Management, Rajendranagar, Hyderabad-500030, India
*Corresponding author
Abstract:

A soil survey was carried out during 2016-2017 in four predominantly Sugarcane growing districts of Telangana state, viz., Sangareddy, Kamareddy, Nizamabad and Khammam, for the presence of entomopathogenic nematodes (EPNs) from Sugarcane ecosystem. The soil samples collected from Kamareddy district showed the highest occurrence of EPNs in 33.33% of the soil samples, followed by Sangareddy (23.33%), Khammam (16.67%) and Nizamabad (5.55%) districts. The isolates of EPNs were utilized for molecular characterization and identification. The genomic DNA of EPN isolates was extracted and 18S and 28S ITS rDNA region of genomic DNA was amplified by PCR technique using a universal primer. The molecular studies shown the occurrence of Heterorhabditis indica in 41.18% of the soil samples, followed by Heterorhabditidoides sp. in 23.53% and Heterorhabditis bacteriophora in 17.65% of the soil samples. The results concluded that H. indica was dominant EPN species occurring in Sugarcane ecosystem. The new EPN species Heterorhabditidoides spp. was reported for the first time in India.


Keywords: Soil survey, Telangana state, Molecular characterization, Heterorhabditidoides
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How to cite this article:

Amit. U. Paschapur, K. Vijaya Lakshmi, Khushbu Chauhan, B. S. Sunanda, Chetna Mathur, Chaitra Bhat, Vishal Somavanshi, Uma Rao and Vinod Pawar. 2018. Report of Novel Entomopathogenic Nematode (Heterorhabditidoides sp.) in Telangana state, India.Int.J.Curr.Microbiol.App.Sci. 7(12): 3611-3620. doi: https://doi.org/10.20546/ijcmas.2018.712.409