National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Aromatic rice is profitable farming venture and having high consumer demand. The main concerns about aromatic rice are low yield and susceptibility to pests and diseases. A rapid and reproducible genetic transformation protocol was optimized for three aromatic rice varieties using the established plant regeneration protocol. Mature embryos were inoculated with Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1305.1 with GUS (reporter gene) and hpt (hygromycin resistance) gene and the transformation experiment was performed by optimizing the parameters infection time and co cultivation periods. Callus induction was obtained when the seeds namely Pusa Basmati 1, (58.6%) Pusa Sugandh 4(56%) and Pusa Sugandh 5 (55.6%) were cultured on MS medium supplemented with 2.5 mg l-1 IAA + 0.5 mg l-1 kinetin + 1.0 gl-1 CH + 30 g l-1 maltose + 100 ml-1 coconut milk and gelled with agar under dark condition. Maximum regeneration was obtained with MS supplemented with 2.5 mg l-1 BAP + 0.5 mg l-1 K + 1.0 mg l-1 K + 1.0 gl-1 CH + 30 g l-1 maltose + 100 ml-1 coconut milk and 0.8 % agar. It was found that hygromycin upto 30μgl-1 was effective for selection of transformants. Putative transformants as indicated by hygromycin test were subjected to GUS assay. GUS activities were found in rice calli after cocultivation. PCR was performed to analyse the transformants.
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