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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 3458-3487
DOI: https://doi.org/10.20546/ijcmas.2018.712.394


Genetic Transformation in Aromatic Indica Rices Mediated by Agrobacterium tumefaciens
N. Aananthi1 and C.R. Anandakumar2
1Department of Plant Breeding and Genetics, AC &RI, TNAU, Killikulam, India
2Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
*Corresponding author
Abstract:

Aromatic rice is profitable farming venture and having high consumer demand. The main concerns about aromatic rice are low yield and susceptibility to pests and diseases. A rapid and reproducible genetic transformation protocol was optimized for three aromatic rice varieties using the established plant regeneration protocol. Mature embryos were inoculated with Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1305.1 with GUS (reporter gene) and hpt (hygromycin resistance) gene and the transformation experiment was performed by optimizing the parameters infection time and co cultivation periods. Callus induction was obtained when the seeds namely Pusa Basmati 1, (58.6%) Pusa Sugandh 4(56%) and Pusa Sugandh 5 (55.6%) were cultured on MS medium supplemented with 2.5 mg l-1 IAA + 0.5 mg l-1 kinetin + 1.0 gl-1 CH + 30 g l-1 maltose + 100 ml-1 coconut milk and gelled with agar under dark condition. Maximum regeneration was obtained with MS supplemented with 2.5 mg l-1 BAP + 0.5 mg l-1 K + 1.0 mg l-1 K + 1.0 gl-1 CH + 30 g l-1 maltose + 100 ml-1 coconut milk and 0.8 % agar. It was found that hygromycin upto 30μgl-1 was effective for selection of transformants. Putative transformants as indicated by hygromycin test were subjected to GUS assay. GUS activities were found in rice calli after cocultivation. PCR was performed to analyse the transformants.


Keywords: Agrobacterium, Aromatic indica rices, Genetic transformation, immature embryos
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How to cite this article:

Aananthi, N. and Anandakumar, C.R. 2018. Genetic Transformation in Aromatic Indica Rices Mediated by Agrobacterium tumefaciens.Int.J.Curr.Microbiol.App.Sci. 7(12): 3458-3487. doi: https://doi.org/10.20546/ijcmas.2018.712.394