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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 702-706
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.081


Importance of EDTA in the Detection of Metallo Beta Lactamase from Imipenem Resistant Gram Negative Bacilli
P. Kanchanadevi1* and S. Chandra Sekaran2
1CSI College of Dental Sciences and Research, Eastveli Street, Madurai, India
2NIMRA Institute of Medical Sciences, Jupidi village, Ibrahimpatnam, Mandal, Krishna DT, Vijaywada, Andhrapradesh, India
*Corresponding author
Abstract:

Gram negative Bacilli isolates resistant to imipenem were screened phenotypically for  Metallo Beta Lactamase( MBL) production by EDTA Disc Synergy (EDS) method and MIC reduction test. EDTA, a chelating agent which inhibits the production of MBL and hence incorporation of EDTA to the resistant strains, results in the zone formation. In this study, Minimal Inhibitory Concentration for Imipenem was also estimated and the incorporation of EDTA to the drug dilution plates differentiated all MBL producing Gram negative Bacilli.


Keywords: MBL(Metallo Beta Lactamase),MIC   EDTA  (Ethylene DiamineTetra Aceticacid).
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How to cite this article:

Kanchanadevi, P., and S. Chandra Sekaran. 2016. Importance of EDTA in the Detection of Metallo Beta Lactamase from Imipenem Resistant Gram Negative Bacilli.Int.J.Curr.Microbiol.App.Sci. 5(11): 702-706. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.081