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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 3346-3357
DOI: https://doi.org/10.20546/ijcmas.2018.712.385


Variability in Total Glucosinolate Content in F2 Generation of B. juncea and their Molecular Validation
V.V. Singh*, Ibandalin Mawlong, Monika Dubey, Pawan Paliwal and M.S. Sujith Kumar
ICAR-Directorate of Rapeseed Mustard Research, Bharatpur, 321303, Rajasthan, India
*Corresponding author
Abstract:

To increase the utilization of seed meal and to improve the feed palatability it is necessary to develop low glucosinolate mustard lines. The present study was intended to develop low glucosinolate varieties in B.juncea through evaluation of variability in total glucosinolate among parental and F2 population. Crosses between NRCHB101×PDZ-1, NRCHB101×RLC-3, NRCHB101×HEERA, DRMR150-35×PDZ-1, DRMR150-35×RLC-3 and NRCHB101×HEERA generated 252 F2 populations. These F2 populations showed the frequency of distribution of total glucosinolate to range from18 µmol/g to 168 µmol/g which is out of the range of parental lines. Clustering of all the progenies derived from the crosses showed considerable diversity in their total glucosinolate content. 8 Lines derived from crosses DRMR150-35×PDZ-1 and NRCHB101× RLC-3 having total glucosinolate content less than 30 µmol/g were reconfirmed by molecular markers.


Keywords: Brassica juncea, Total glucosinolate, Molecular markers, F2 populations
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How to cite this article:

Singh, V.V., Ibandalin Mawlong, Monika Dubey, Pawan Paliwal and Sujith Kumar, M.S. 2018. Variability in Total Glucosinolate Content in F2 Generation of B. juncea and their Molecular Validation.Int.J.Curr.Microbiol.App.Sci. 7(12): 3346-3357. doi: https://doi.org/10.20546/ijcmas.2018.712.385