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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 637-647
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.075


Molecular Characterization of Carbapenem Resistant Acinetobacter baumannii in Cancer Patients
Rasha M. Abdel Hamid1*, Safaa S. Hassan1, Hadir A. El-Mahallawy1 and Magdy Saber2
1Clinical pathology department, National Cancer Institute, Cairo University, Egypt
2Medical Oncology Department, National Cancer Institute, Cairo University, Egypt
*Corresponding author
Abstract:

This study aimed to investigate the prevalence of Multidrug resistant Acinetobacter baumannii (MDR-AB) as a cause of health-care associated infection (HAI) in cancer patients and study the mechanisms of carbapenem resistance in relation to different risk factors. In this study, Multidrug resistant Acinetobacter were isolated and subjected to Imipenem-EDTA combined disk synergy (CDST-IPM), multiplex PCR to detect four families of OXA-type carbapenamases and sequencing of blaOXA-51 like gene. During one and a half year, MDR-AB accounted for 15% of gram negative pathogens. The majority of MDR-AB infections were in patients who spent 7 or more days in the hospital (76.8%). More than one mechanism of resistance were detected, as metallo-β-lactamase (MBL) producers, blaOXA-51 like and blaOXA-23 like were detected in 80%, 94% and 63% of MDR-AB; respectively. The blaOXA-23 and MBLs coexisted simultaneously in 71% of cases (p=0.003); and 7% of imipenem resistant isolates were negative for MBL and blaOXA-23 suggesting other resistance mechanisms. The presence of Acinetobacter resistant genes (blaOXA-51 and blaOXA-23) was significantly associated with carbapenem use, p=0.015 and 0.064; respectively. The phylogenetic analysis showed different genetic variants. In conclusion, acquisition of carbapenem resistance among MDR-AB could be related to carbapenem therapy and prolonged duration of hospitalization.


Keywords: Multidrug resistance Acinetobacter baumannii- carbapenem resistance- Metallo-β-lactamase producers- OXA-type carbapenamases
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How to cite this article:

Rasha M. Abdel Hamid, Safaa S. Hassan, Hadir A. El-Mahallawy and Magdy Saber. 2016. Molecular Characterization of Carbapenem Resistant Acinetobacter baumannii in Cancer Patients.Int.J.Curr.Microbiol.App.Sci. 5(11): 637-647. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.075