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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2762-2771
DOI: https://doi.org/10.20546/ijcmas.2018.712.313


Phenotypic and Molecular Characterization of Carbapenamase among Acinetobacter Isolates in a Tertiary Care Hospital, Tamil Nadu
Thiriveni1*, U. Umadevi2 and N. Lakshmipriya2
1Coimbatore Medical College, Coimbatore – 641 014, Tamil Nadu, India
2Institute of Microbiology, Madras Medical College, Chennai – 600 003, Tamil Nadu, India
*Corresponding author
Abstract:

Acinetobacter the aerobic non fermenting GNCB are emerging as important pathogens in health care associated infection, exhibiting increased antimicrobial resistance by virtue of multiple resistant mechanisms with practically no therapeutic options. The objective was to determine the multitude of resistant mechanisms by phenotypic and genotypic characterization and the prevalent antimicrobial susceptibility pattern. 175 clinically significant non-duplicate Acinetobacter isolates were included. ESBL, AmpC and carbapenamase production were determined by CLSI phenotypic confirmatory method, AmpC disc test, Modified Hodge test and Imipenem-EDTA combined disc test respectively. Presence of genes conferring carbapenem resistance viz OXA-23, blaVIM1 & blaIMP1 were determined by PCR. A. baumannii (81.14%) was the most common species with significant difference (p value <0.05) in antimicrobial sensitivity pattern. MDR was 60%, XDR was 11.43% and no PDR isolate. Carbapenem resistance was detected in 20 isolates (11.43%). MIC of meropenem for the resistant isolates were between 32µg/ml and 256µg/ml. MHT was positive in 9 (45%) isolates and IEDT was positive in 9(45%) isolates. 61(34.86%) isolates were ESBL producers and 23(13.14%) isolates were AmpC producers. On molecular characterization OXA-23 gene was detected in all 20 meropenem resistant isolates (100%). MBL conferred by blaVIM1 gene was positive in 9 isolates (45%) and blaIMP1 in 7 isolates (35%). Even though genotypic methods are the gold standard, phenotypic methods due to ease of performance and cost effectiveness still remain useful for characterization of carbapenemase resistance.


Keywords: MDR- Multi drug resistant, XDR- Extended drug resistant, PDR- Pan drug resistant, ESBL- Extended spectrum beta lactamase, MHT- Modified Hodge test, IEDT- Imipenem EDTA combined disc test
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How to cite this article:

Thiriveni, J., U. Umadevi and Lakshmipriya, N. 2018. Phenotypic and Molecular Characterization of Carbapenamase among Acinetobacter Isolates in a Tertiary Care Hospital, Tamil Nadu.Int.J.Curr.Microbiol.App.Sci. 7(12): 2762-2771. doi: https://doi.org/10.20546/ijcmas.2018.712.313