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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 594-603
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.070


Development of Herbal-Silver Nano Composite (HSNC): Antibacterial Evaluation and Investigation of fnb A and fnb B Genes Coding Fibronectin-binding Proteins in Staphylococcus aureus
J. Meenakshi* and V. Kalai Gandhi
Department of Microbiology, Dr. GRD College of Science and Commerce,Coimbatore-641014, Tamil Nadu, India,
*Corresponding author
Abstract:

A novel composite synthesized using silver nanoparticles with Terminalia chebula extracts was selected as the primary objective of this present research. Also an investigation on the virulence genes, fnbA and fnbB that regulate the production of the fibronectin binding proteins in S. aureus using polymerase chain reaction was studied. Silver nanoparticles was synthesized and a novel composite was developed using medically significant Terminalia chebulaextracts in three different ratios(1:1, 1:2 and 2:1). Antibacterial activity of the developed herbal-silver nanocomposite (HSNC) against the seven Staphylococcus aureus strains was determined. The presence of genes encoding adhesin specific fibronectin-binding proteins of fnbA and fnbBin the selected Staphylococus aureus strain-7 was investigated using standard polymerase chain reaction method. The obtained inhibitory clear zones range from 38mm to 49mm for all the ratios against all the test strains. Maximum inhibition clear zone (ICZ) of 49mm was observed for three different ratios, 1:1, 1:2 and 2:1 against test strains- 1, 5 and 7 respectively; and the lowest of 38mm for all the ratios against test strains-1, 2, 3 and 4 respectively. In the selected strain (S. aureus 7), both fnbA and fnbB genes were detected. This was evident and proved by comparing the electrophoretic DNA patterns of the reference strain, Staphylococcus aureus NCTC 8325. The obtained results were considered to be as a preliminary experiment for the development of a novel pharmacological product in future.


Keywords: Silver ions, Composite, Fibronectin, Inhibition clear zone, fnbA and fnbB.
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How to cite this article:

Meenakshi, J., and Kalai Gandhi, V. 2016. Development of Herbal-Silver Nano Composite (HSNC): Antibacterial Evaluation and Investigation of fnb A and fnb B Genes Coding Fibronectin-binding Proteins in Staphylococcus aureusInt.J.Curr.Microbiol.App.Sci. 5(11): 594-603. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.070