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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:12, December, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2392-2400
DOI: https://doi.org/10.20546/ijcmas.2018.712.271


Life Cycle of Cereal Cyst Nematode, Heterodera avenae on Resistant and Susceptible Barley Cultivars
Anshul Chhachhia* and R.S. Kanwar
Department of Nematology, CCS Haryana Agricultural University, Hisar-125004, India
*Corresponding author
Abstract:

The development of Heterodera avenae was studied on four resistant cultivars (BH 393, BH959, RD 2035 and DWRB 91) and one susceptible cultivar (BH902). After 20 days of germination inoculation was done with second stage juveniles @ 250 J2 / pot. Plants of each cultivar were uprooted at 15 days interval after the inoculation and observations were taken till adults were formed. Roots of uprooted plants were stained with 0.1% acid fuchs in lactophenol and observations on the development stages were taken by mounting the nematode dissected out of root in lactophenol. Penetration of the J2 of H. avenae was occurred insusceptible as well as in resistant cultivars but penetration was more in susceptible cultivars than resistant cultivars. In all the varieties, almost all the J2 could reach the fourth stage. Infected roots exhibited only slight swelling at the site of infection. H. avenae completed life cycle from J2 to female in 75 days on all varieties except, on RD 2035. Maximum number of cysts and cyst content was found in susceptible variety than resistant varieties but on RD 2035 no cysts were formed.


Keywords: Heterodera avenae, Barley, Life cycle, Penetration and Development

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How to cite this article:

Anshul Chhachhia and Kanwar, R.S. 2018. Life Cycle of Cereal Cyst Nematode, Heterodera Avenae on Resistant and Susceptible Barley Cultivars.Int.J.Curr.Microbiol.App.Sci. 7(12): 2392-2400. doi: https://doi.org/10.20546/ijcmas.2018.712.271
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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