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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2377-2384
DOI: https://doi.org/10.20546/ijcmas.2018.712.269


Isolation, Identification and Characterization of Enteric Bacteria from Post Weaning Diarrheic Pigs and their Resistance to Multiple Antibiotics
Dinesh Mittal1, Kushal Grakh1, Anand Prakash1*, Pallavi Moudgil1, Bhanita Devi2 and Vijay Jadhav1
1Department of Veterinary Public Health and Epidemiology
2Department of Animal Biotechnology, LUVAS, Hisar, Haryana, India
*Corresponding author
Abstract:

In the present study, 25 diarrheic faecal samples/ rectal swabs collected from post weaning pigs were examined by cultural method for the presence of Enterobacteriaceae members which resulted in isolation of 18 lactose fermenter and two non-lactose fermenter bacterial colonies. The recovered colonies were subjected to VITEK®2 Compact System for the identification of bacteria. Out of 18 lactose fermenter bacterial colonies, 15 were identified as Escherichia coli, two as Enterobacter asburiae, and one as Klebsiella pneumoniae. Both the lactose non-fermenter bacterial colonies were identified as Pseudomonas aeruginosa. In-vitro antibiotic sensitivity pattern of E. coli isolates showed 100% sensitivity to chloramphenicol, gentamicin and ampicillin/sulbactam whereas 60% isolates were found to be resistant to co-trimoxazole and 53% isolates to amoxicillin clavulanate and cefotaxime. All E. coli isolates showed resistance to more than four antibiotics, four isolates showed resistance to six antibiotics and two isolates showed resistance to more than seven antibiotics. Isolates of K. pneumoniae, Enterobacter asburiae and P. aeruginosa found to be sensitive to most of the antibiotics tested; whereas these isolates showed resistance to amoxycillin/clavulanate and co-trimoxazole. This study helps in assessment of bacterial resistance of enteric bacteria recovered from diarrheic pigs.


Keywords: Antibiotic resistance, E. coli, Post weaning diarrhea, Swine production, VITEK®2
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How to cite this article:

Dinesh Mittal, Kushal Grakh, Anand Prakash, Pallavi Moudgil, Bhanita Devi and Vijay Jadhav. 2018. Isolation, Identification and Characterization of Enteric Bacteria from Post Weaning Diarrheic Pigs and their Resistance to Multiple Antibiotics.Int.J.Curr.Microbiol.App.Sci. 7(12): 2377-2384. doi: https://doi.org/10.20546/ijcmas.2018.712.269