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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2215-2221
DOI: https://doi.org/10.20546/ijcmas.2018.712.252


Isolation and Evaluation of Cellulolytic Yeasts for Production of Ethanol from Wheat Straw
Geeta Shirnalli, M. Ashwini* and Dhanashree Gachhi
AICRP ON EAAI (Bioconversion Technology), Main Agriculture Research Station, University of Agricultural Sciences, Dharwad-580 005, Karnataka, India
*Corresponding author
Abstract:

The cellulolytic efficiency of yeast isolates obtained from different fruit and fruit wastes was evaluated in terms of degradation of cellulose, release of reducing sugars and bioethanol production. The isolates CY-3, CY-4, CY-8 and CY-15 together with a reference cellulolytic strain Saccharomyces cerevisiae (NCIM 3220) displayed maximum hydrolysis of carboxy methyl cellulose (CMC) to yield reducing sugars when determined in 2 days interval for up to 22 days, Based on this, the isolates were further evaluated for combined saccharification and fermentation of a pretreated lignocellulosic substrate such as wheat straw in flask culture studies. The concentration of ethanol produced by the cellulolytic yeasts at the end of fermentation was determined by Gas Chromatography. Strain CY-15 produced 0.573g/L of ethanol which was on par with the reference strain which produced 0.68 g/L. Thus the study attempts to develop a sustainable bioethanol production technology by simultaneous saccharification and fermentation of wheat straw using native yeasts.


Keywords: Cellulolytic yeasts, Fruit wastes, Ethanol, Saccharification, Lignocellulosic waste
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How to cite this article:

Geeta Shirnalli, Ashwini, M. and Dhanashree Gachhi. 2018. Isolation and Evaluation of Cellulolytic Yeasts for Production of Ethanol from Wheat Straw.Int.J.Curr.Microbiol.App.Sci. 7(12): 2215-2221. doi: https://doi.org/10.20546/ijcmas.2018.712.252