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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2157-2163
DOI: https://doi.org/10.20546/ijcmas.2018.712.244


Biofilm Detection and Clinical Significance of Staphylococcus epidermidis Isolates in a Tertiary Care Hospital, Karimnagar, India
Kondal Rao Ravilla1, Amar C. Sajjan1* and Sachin Gurnule2
1Department of Microbiology
2Department of Community Medicine, CAIMS Karimnagar, India
*Corresponding author
Abstract:

Aims of the study are to detect biofilm producing Staphylococcus epidermidis isolated from various clinical specimens. Total 73 Staphylococcus epidermidis isolates were collected from clinical samples like blood, post-operative wound swabs, IV catheter tips, catheterized urine, and exudates received from various clinical departments. The study was carried out over a period of one year. The specimens received were processed by conventional methods. Tissue Culture plate method was used for detection of biofilm. IV catheter tip samples revealed 25%, implant device associated infections revealed 20%, the Catheterized urine samples showed 17%, blood culture 6%, ventilator associated infections 20%, post-operative wound infections 13.29% and exudates 3.33% of Staphylococcus epidermidis isolates. Isolates with O. D. values more than 0.2 were considered as high biofilm producers. 52.1% of S. epidermidis isolates were weak biofilm producers, 24.66% were moderate biofilm producers and 20.54% were high biofilm producers. Isolates from IV catheter tips showed high biofilm formation. Increase in use of implant devices, unnecessary and prolonged use of urinary catheter and IV catheters can lead to biofilm formation which pose difficulty in treating and eradicating them.


Keywords: Coagulase negative Staphylococci, Biofilm, Staphylococcus epidermidis
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How to cite this article:

Kondal Rao Ravilla, Amar C. Sajjan and Sachin Gurnule. 2018. Biofilm Detection and Clinical Significance of Staphylococcus epidermidis Isolates in a Tertiary Care Hospital, Karimnagar, India.Int.J.Curr.Microbiol.App.Sci. 7(12): 2157-2163. doi: https://doi.org/10.20546/ijcmas.2018.712.244