Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2148-2156
DOI: https://doi.org/10.20546/ijcmas.2018.712.243


Detection of Extended Spectrum Β-Lactamase (ESBL) and Metallo Β-Lactamase (MBL) In Pseudomonas aeruginosa and Acinetobacter Species
Ajana Rai*
Department of Microbiology, Kathmandu College of Science and Technology, Tribhuwan University, Kamalpokhari, Kathmandu, Nepal
*Corresponding author
Abstract:

Infections caused by multidrug-resistant bacteria expressing 𝛽-lactamases pose serious challenges to clinicians worldwide because these bacteria are resistant to a broad range of 𝛽-lactams. This study was undertaken to detect extended spectrum β-lactamases (ESBL) and metallo‑beta‑lactamase (MBL) producing Pseudomonas aeruginosa and Acinetobacter species from wound pus and sputum samples. A prospective study was performed over a period of 8 months in a tertiary care hospital. A total of 17 clinical isolates of P. aeruginosa and 18 of Acinetobacter species were tested for the presence of ESBL, and metallo‑beta‑lactamase enzyme. Detection of ESBL was done by the Cephalosporins/Clavulanate Combination Disk method as per Clinical and Laboratory Standards Institute (CLSI) guidelines, and MBL was detected by Imipenem - EDTA Combined disk synergy test. Among 17 isolates of P. aeruginosa, 64.7% were MDR, 41.17 % of were positive for ESBL while 35.2% were MBL producers. Among 18 isolates of Acinetobacter spp, 44.4% were MDR, 16.6% were ESBL producers while 44.4 % were MBL‑producing species. A high prevalence of MDR, ESBL, MBL positive isolates among these non-fermentating bacteria is increasing at an alarming rate. Thus, proper antibiotic policy and measures to restrict the indiscriminative use of cephalosporins and carbapenems should be taken to minimize the emergence of this multiple beta‑lactamase‑producing pathogens.


Keywords: Pseudomonas aeruginosa, Acinetobacter spp, Multidrug Resistance, ESBL, MBL
Download this article as Download

How to cite this article:

Ajana Rai. 2018. Detection of Extended Spectrum Β-Lactamase (ESBL) and Metallo Β-Lactamase (MBL) In Pseudomonas aeruginosa and Acinetobacter Species.Int.J.Curr.Microbiol.App.Sci. 7(12): 2148-2156. doi: https://doi.org/10.20546/ijcmas.2018.712.243