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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1907-1914
DOI: https://doi.org/10.20546/ijcmas.2018.712.221


Use of Microsatellite Markers for Assessing Genetic Variability in Wheat Genotypes for Yellow Rust Resistance
Meenakshi Rathi*, Mukesh Kumar, Vijay Kumar, Pooja and Lakshmi Chaudhary
Department of Genetics and Plant Breeding, College of Agriculture Chaudhary Charan Singh Haryana Agricultural University, Hisar 125001, India
*Corresponding author
Abstract:

A set of sixty microsatellite markers were used to characterize 49 genotypes of wheat. Out of these, 23 markers showed polymorphism with a total of 170 alleles. Average number of SSR alleles per locus was 2.88 and ranges from 2 (Cfa2040, Xgwm261, Xgwm382, Xgwm413, Xgwm170, Wmx44, Cslv34, Xgwm501, Xgwm70, Barc136, Barc101, Xgwm352, Xgwm130, Barc187, Barc76, Wmc407) to 5 (Xgwm190). The PIC value varied from 0.11-0.65 and 7 out of 23 SSR loci, Xgwm261, Xgwm295, Xgwm190, Xgwm46, Gwm11, Xgwm273 and Xsps3000 had PIC value more than 0.5 thus considered very useful in estimating genetic diversity of the genotypes. The UPGMA cluster grouped into two, major cluster 1 consists of 6 sub clusters whereas major cluster 2 was not further fragmented and had only one genotype WH1191. Cluster pattern revealed that, sub-cluster 2 was the largest consisting maximum number of 24 genotypes. This cluster analysis grouped genotypes on the basis of similarity at DNA level, thus for selecting parents for hybridization program against yellow rust disease can be chosen from different clusters.


Keywords: Microsatellite, Yellow rust, Diversity, PIC and UPGMA cluster
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How to cite this article:

Meenakshi Rathi, Mukesh Kumar, Vijay Kumar, Pooja and Lakshmi Chaudhary. 2018. Use of Microsatellite Markers for Assessing Genetic Variability in Wheat Genotypes for Yellow Rust Resistance.Int.J.Curr.Microbiol.App.Sci. 7(12): 1907-1914. doi: https://doi.org/10.20546/ijcmas.2018.712.221